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dc.contributor.authorStotz, Henrik
dc.contributor.authorHertel, R.
dc.identifier.citationStotz , H & Hertel , R 1994 , ' Re-evaluation of the role of auxin binding site II ' , Journal of Plant Growth Regulation , vol. 13 , no. 2 , pp. 79-85 .
dc.identifier.otherPURE: 1442597
dc.identifier.otherPURE UUID: 1efd7f25-af05-4889-8e1d-502f0dff23eb
dc.identifier.otherScopus: 0008925408
dc.descriptionCopyright 2007 Elsevier B.V., All rights reserved.
dc.description.abstractBinding of 1-naphthylacetic acid (1-NAA) was assayed in microsomal membranes from Zea mays coleoptiles and from hypocotyls of Cucurbita pepo. Auxin binding site II was differentiated from site I binding by using phenylacetic acid (PAA) to saturate site I binding capacity. The amount of type-II binding sites, per gram original fresh weight, was 34 pmol with Zea and 6.4 pmol with Cucurbita. When maize membranes were separated by dextran gradient centrifugation, auxin binding site II migrated coincident with tonoplast marker enzymes. The physiologically active auxin 4-chloroindoleacetic acid (4-Cl-IAA) competed very poorly with 1-NAA binding to both site I and site II. This result suggests that sites I and II are not involved in the regulation of growth. When comparing isolated outer epidermis with intact coleoptile of Zea, similar amounts and ratios of site I and site II binding activities were observed.en
dc.relation.ispartofJournal of Plant Growth Regulation
dc.titleRe-evaluation of the role of auxin binding site IIen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop Protection and Climate Change
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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