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dc.contributor.authorFord, Robert J.
dc.contributor.authorBarker, Amy M.
dc.contributor.authorBakker, Saskia E.
dc.contributor.authorCoutts, Robert H.A.
dc.contributor.authorRanson, Neil A.
dc.contributor.authorPhillips, Simon E. V.
dc.contributor.authorPearson, Arwen R.
dc.contributor.authorStockley, Peter G.
dc.date.accessioned2013-07-29T10:18:23Z
dc.date.available2013-07-29T10:18:23Z
dc.date.issued2013-03-25
dc.identifier.citationFord , R J , Barker , A M , Bakker , S E , Coutts , R H A , Ranson , N A , Phillips , S E V , Pearson , A R & Stockley , P G 2013 , ' Sequence-specific, RNA-protein interactions overcome electrostatic barriers preventing assembly of satellite tobacco necrosis virus coat protein ' , Journal of Molecular Biology , vol. 425 , no. 6 , pp. 1050-64 . https://doi.org/10.1016/j.jmb.2013.01.004
dc.identifier.issn1089-8638
dc.identifier.otherPURE: 1908793
dc.identifier.otherPURE UUID: 9e450dc4-493a-4103-a214-a28ff0f29ffd
dc.identifier.otherPubMed: 23318955
dc.identifier.otherScopus: 84875429396
dc.identifier.urihttp://hdl.handle.net/2299/11200
dc.descriptionCopyright © 2013 Elsevier Ltd. All rights reserved.
dc.description.abstractWe have examined the roles of RNA-coat protein (CP) interactions in the assembly of satellite tobacco necrosis virus (STNV). The viral genomic RNA encodes only the CP, which comprises a β-barrel domain connected to a positively charged N-terminal extension. In the previous crystal structures of this system, the first 11 residues of the protein are disordered. Using variants of an RNA aptamer sequence isolated against the CP, B3, we have studied the sequence specificity of RNA-induced assembly. B3 consists of a stem-loop presenting the tetra-loop sequence ACAA. There is a clear preference for RNAs encompassing this loop sequence, as measured by the yield of T=1 capsids, which is indifferent to sequences within the stem. The B3-containing virus-like particle has been crystallised and its structure was determined to 2.3Å. A lower-resolution map encompassing density for the RNA has also been calculated. The presence of B3 results in increased ordering of the N-terminal helices located at the particle 3-fold axes, which extend by roughly one and a half turns to encompass residues 8-11, including R8 and K9. Under assembly conditions, STNV CP in the absence of RNA is monomeric and does not self-assemble. These facts suggest that a plausible model for assembly initiation is the specific RNA-induced stabilisation of a trimeric capsomere. The basic nature of the helical extension suggests that electrostatic repulsion between CPs prevents assembly in the absence of RNA and that this barrier is overcome by correct placement of appropriately orientated helical RNA stems. Such a mechanism would be consistent with the data shown here for assembly with longer RNA fragments, including an STNV genome. The results are discussed in light of a first stage of assembly involving compaction of the genomic RNA driven by multiple RNA packaging signal-CP interactions.en
dc.format.extent15
dc.language.isoeng
dc.relation.ispartofJournal of Molecular Biology
dc.subjectTobacco necrosis satellite virus
dc.subjectModels, Molecular
dc.subjectCapsid Proteins
dc.subjectCapsid
dc.subjectMolecular Sequence Data
dc.subjectGenome, Viral
dc.subjectAmino Acid Sequence
dc.subjectRNA, Viral
dc.subjectNucleic Acid Conformation
dc.subjectProtein Conformation
dc.subjectBinding Sites
dc.titleSequence-specific, RNA-protein interactions overcome electrostatic barriers preventing assembly of satellite tobacco necrosis virus coat proteinen
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dcterms.dateAccepted2013-03-25
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.jmb.2013.01.004
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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