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dc.contributor.authorSlater, R. J.
dc.contributor.editorWalker, John M.
dc.identifier.citationSlater , R J 1985 , The purification of poly(a)-containing RNA by affinity chromatography . in J M Walker (ed.) , Nucleic Acids . Methods in Molecular Biology , vol. 2 , Humana Press / Springer , pp. 117-20 .
dc.identifier.otherPURE: 2481495
dc.identifier.otherPURE UUID: 133368cf-68fb-45f8-8224-1b880278d93c
dc.identifier.otherPubMed: 21374180
dc.description.abstractThe vast majority of eukaryotic mRNA molecules contain tracts of poly(adenylic) acid, up to 250 bases in length, at the 3' end. This property is very useful from the point of view of mRNA extraction because it forms the basis of a convenient and simple affinity chromatography procedure (1). Under high salt conditions (0.3-0.5M NaCl or KCl), poly(A) will hybridize to oligo(dT)-cellulose or poly(U)-Sepharose. These commercially available materials consist of polymers of about 10-20 nucleotides, covalently bound to a carbohydrate support, and bind RNA containing a poly(A) tract as short as 20 residues. Ribosomal and transfer RNAs do not possess poly(A) sequences and will not bind (see Note 1).en
dc.publisherHumana Press / Springer
dc.relation.ispartofNucleic Acids
dc.relation.ispartofseriesMethods in Molecular Biology
dc.titleThe purification of poly(a)-containing RNA by affinity chromatographyen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionMicrobiology and Biotechnology
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.description.statusNon peer reviewed
dc.relation.schoolSchool of Life and Medical Sciences

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