Complete nucleotide sequences of four dsRNAs associated with a new chrysovirus infecting Aspergillus fumigatus
Bignell, Elaine M.
Coutts, Robert H.A.
A new double-stranded RNA (dsRNA) virus designated A. fumigatus chrysovirus (AfuCV), belonging to the family Chrysoviridae, has been identified in the filamentous fungus Aspergillus fumigatus. The virus was detected in five of 390 A. fumigatus isolates screened. Analysis of purified dsRNA revealed four distinct species 3560, 3159, 3006 and 2863 base pairs in length (dsRNAs 1-4) which were cloned and sequenced. Each dsRNA contains a single open reading frame (ORF) with short 5' and 3' untranslated regions containing strictly conserved termini. The deduced 1114 amino acid (aa) protein (molecular mass=128 kDa) encoded by the dsRNA1 ORF showed homology to the RNA-dependent RNA polymerase (RdRP) of viruses belonging to the Chrysoviridae. Eight motifs characteristic of RdRPs were identified. The dsRNA2 ORF encodes the putative coat protein subunit (953aa; molecular mass=107 kDa). The dsRNA3 and dsRNA4 ORFs respectively encode putative proteins (891aa, molecular mass=99 kDa) and (847aa, molecular mass=95 kDa), both of which have significant similarity to proteins encoded by comparable chrysovirus dsRNAs. The dsRNA profile, amino acid sequence alignments, and phylogenetic analyses all indicate that AfuCV is a new species within the family Chrysoviridae.
Published inVirus Research
MetadataShow full item record
Showing items related by title, author, creator and subject.
Lyon, C.; Dickerson, R. (Chinese Information Processing Society of China, 1999)Characteristics of natural language can be illuminated through the application of well known tools in Information Theory. This paper shows how some of these characteristics can be exploited in the development of automated ...
Children retain implicitly learned phonological sequences better than adults: A longitudinal study Smalle, Eleonore H M; Page, Michael; Duyck, Wouter; Edwards, Martin G.; Szmalec, Arnaud (2018-09-01)Whereas adults often rely on explicit memory, children appear to excel in implicit memory, which plays an important role in the acquisition of various cognitive skills, such as those involved in language. The current study ...
The nucleotide sequence of satellite tobacco necrosis virus strain C and helper-assisted replication of wild-type and mutant clones of the virus Bringloe, D.H.; Pelpel, M.; Coutts, Robert H.A.; Gultyaev, A.P.; Pleij, C.W.A. (1998-06)The complete nucleotide sequence of satellite tobacco necrosis virus strain C (STNV-C) was determined. The genome has a similar overall organization to two STNV isolates studied previously but differs significantly from ...