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        Development of PCR based diagnostic techniques for the two mating types of Pyrenopeziza brassicae (light leaf spot) on winter oilseed rape (Brassica napus ssp. oleifera)

        Author
        Foster, S.J.
        Singh, G.
        Fitt, Bruce D.L.
        Ashby, A.M.
        Attention
        2299/11405
        Abstract
        Two sets of PCR primers were designed from DNA sequence which flanked the 3' end of the Pyrenopeziza brassicae mating type idiomorphs (MAT-1 and MAT-2). The first set of primers facilitated the amplification of a 750 bp product from genomic DNA of 50 isolates of P. brassicae, which included both mating types. The 750 bp PCR product was also amplified from DNA extracted from Brassica napus tissue infected with P. brassicae. The 750 bp PCR product was not produced when these diagnostic primers were used in PCR reactions containing DNA from a number of other fungal species, including fungal pathogens of oilseed rape and Tapesia yallundae, which is closely related to P. brassicae. The second set of primers were designed to amplify different sized products from the two mating types of P. brassicae. These mating type specific primers facilitated the amplification of a 440 bp product from all MAT-1 isolates tested and a 535 bp product from 30% of the MAT-2 isolates tested. The value of molecular diagnostic techniques for the identification of P. brassicae in oilseed rape crops, for distinguishing between mating types of the fungus and in disease forecasting is discussed. (C) 1999 Academic Press.
        Publication date
        1999-08
        Published in
        Physiological and Molecular Plant Pathology
        Published version
        https://doi.org/10.1006/pmpp.1999.0207
        Other links
        http://hdl.handle.net/2299/11405
        Metadata
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        Related items

        Showing items related by title, author, creator and subject.

        • Interactions in the Brassica napus-Pyrenopeziza brassicae pathosystem and sources of resistance to P. brassicae (light leaf spot) 

          Karandeni Dewage, Chinthani Shanika; Qi, Aiming; Stotz, Henrik U.; Huang, Yongju; Fitt, Bruce D. L. (2021-08-24)
          Abstract: Pyrenopeziza brassicae, cause of light leaf spot (LLS), is an important pathogen of oilseed rape and vegetable brassicas and has a wide geographic distribution. Exploitation of host resistance remains the most ...
        • The roles of ascospores and conidia of Pyrenopeziza brassicae in light leaf spot epidemics on winter oilseed rape (Brassica napus) in the UK 

          Gilles, T.; Fitt, Bruce D.L.; McCartney, H. A.; Papastamati, K.; Steed, J. M. (2001)
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        • Effects of wetness period and temperature on development of dark pod spot (Alternaria brassicae) on oilseed rape (Brassica napus) 

          Hong, C.X.; Fitt, Bruce D.L.; Welham, S.J. (1996-12)
          In controlled environment experiments, when oilseed rape pods or leaves were inoculated with spore suspensions of Alternaria brassicae, the maximum disease incidence (proportion of pods or leaves diseased) increased as ...
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