Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells
Author
Njoh, Kerenza L.
Patterson, Laurence H.
Zloh, Mire
Wiltshire, Marie
Fisher, Janet
Chappell, Sally
Ameer-Beg, Simon
Bai, Yanhong
Matthews, Daniel
Errington, Rachel J.
Smith, Paul J.
Attention
2299/11451
Abstract
We report on the potential DNA binding modes and spectral characteristics of the cell-permeant far red fluorescent DNA dye, DRAQ5, in solution and bound within intact cells. Our aim was to determine the constraints for its use in flow cytometry and bioimaging.