Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells
Njoh, Kerenza L.
Patterson, Laurence H.
Errington, Rachel J.
Smith, Paul J.
We report on the potential DNA binding modes and spectral characteristics of the cell-permeant far red fluorescent DNA dye, DRAQ5, in solution and bound within intact cells. Our aim was to determine the constraints for its use in flow cytometry and bioimaging.