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        Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry disease

        Author
        Kozlakidis, Z.
        Citir, A.
        Acikgoz, S.
        Coutts, Robert H.A.
        Attention
        2299/11473
        Abstract
        A reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Amasya cherry disease (ACD) in naturally infected sweet cherry (Prunus avium) leaves sampled from Turkey. The procedure was based on detection of the presence of a mycoviral-like double-stranded RNA (dsRNA) of 5.3 kbp always found in association with ACD, which is probably caused by a fungus. Specific primers were designed to amplify a fragment of the diagnostic dsRNA. The method will improve routine diagnosis of ACD in Prunus spp.
        Publication date
        2007-12
        Published in
        Plant Pathology
        Published version
        https://doi.org/10.1111/j.1365-3059.2007.01690.x
        Other links
        http://hdl.handle.net/2299/11473
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