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dc.contributor.authorAcikgoez, S.
dc.contributor.authorDoeken, M. T.
dc.contributor.authorDegirmenci, N. F.
dc.contributor.authorCoutts, Robert H.A.
dc.contributor.authorKozlakidis, Z.
dc.date.accessioned2013-09-02T12:45:04Z
dc.date.available2013-09-02T12:45:04Z
dc.date.issued2007-12
dc.identifier.citationAcikgoez , S , Doeken , M T , Degirmenci , N F , Coutts , R H A & Kozlakidis , Z 2007 , ' A modified procedure for isolating double-stranded RNA: Application to diagnosis of Amasya cherry disease ' , Journal of Phytopathology , vol. 155 , no. 11-12 , pp. 743-745 . https://doi.org/10.1111/j.1439-0434.2007.01288.x
dc.identifier.issn0931-1785
dc.identifier.otherPURE: 1953497
dc.identifier.otherPURE UUID: 8467df8f-7aac-4b13-aa56-66536cc21cee
dc.identifier.otherWOS: 000250944600016
dc.identifier.otherScopus: 36248947922
dc.identifier.urihttp://hdl.handle.net/2299/11474
dc.description.abstractA novel method of isolating double-stranded RNA (dsRNA) from cherry leaves affected with Amasya cherry disease (ACD) using silica capture in combination with non-ionic cellulose was developed. Unfractionated RNAs prepared using the new procedure and used to isolate dsRNA was cleaner in terms of contamination with proteins and phenolics when compared with preparations isolated with a phenol/chloroform method. The generated dsRNA was successfully utilized in a reverse transcription-polymerase chain reaction method developed to detect a diagnostic, mycovirus dsRNA element always associated with ACD.en
dc.format.extent3
dc.language.isoeng
dc.relation.ispartofJournal of Phytopathology
dc.subjectRT-PCR
dc.subjectCHLOROTIC RUSTY SPOT
dc.subjectAmasya cherry disease
dc.subjectCOMPLEX PATTERN
dc.subjectPCR
dc.subjectdsRNA
dc.titleA modified procedure for isolating double-stranded RNA: Application to diagnosis of Amasya cherry diseaseen
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop Protection and Climate Change
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1111/j.1439-0434.2007.01288.x
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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