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dc.contributor.authorJiang, Xiaojing
dc.contributor.authorQu, Mingjing
dc.contributor.authorDenholm, Ian
dc.contributor.authorFang, Jichao
dc.contributor.authorJiang, Weihua
dc.contributor.authorHan, Zhaojun
dc.date.accessioned2013-09-03T08:14:59Z
dc.date.available2013-09-03T08:14:59Z
dc.date.issued2009-01-09
dc.identifier.citationJiang , X , Qu , M , Denholm , I , Fang , J , Jiang , W & Han , Z 2009 , ' Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae) ' , Biochemical and Biophysical Research Communications , vol. 378 , no. 2 , pp. 269-272 . https://doi.org/10.1016/j.bbrc.2008.11.046
dc.identifier.issn0006-291X
dc.identifier.otherPURE: 1936377
dc.identifier.otherPURE UUID: edb96dae-9344-4357-87b6-3e628c6e6225
dc.identifier.otherWOS: 000262063100025
dc.identifier.otherScopus: 57049146789
dc.identifier.urihttp://hdl.handle.net/2299/11488
dc.description.abstractTwo full-length genes encoding different acetylcholinesterases (AChEs), designated as Ch-ace1 and Ch-ace2 were cloned from strains of the rice stern borer (Chilo suppressalis) susceptible and resistant to the organophosphate insecticide triazophos. Sequence analysis found an amino acid Mutation A314S in Ch-ace1 (corresponding to A201 in Torpedo californica AChE) that was consistently associated with the occurrence of resistance. This mutation removed an MspA1 I restriction site from the wild type allele. An assay based on restriction fragment length polymorphism (RFLP) analysis was developed to diagnose A314S genotypes in held populations. Results showed a strong correlation between frequencies of the Mutation and phenotypic levels of resistance to triazophos. The assay offers a prospect for rapid monitoring of resistance and assisting with the appropriate choice of insecticide for combating damage caused by C. suppressalis. 2008 Elsevier Inc. All rights reserved.en
dc.format.extent4
dc.language.isoeng
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.subjectPLUTELLA-XYLOSTELLA
dc.subjectGENES
dc.subjectAcetylcholinesterase
dc.subjectChilo suppressalis
dc.subjectGene mutation
dc.subjectAPHIS-GOSSYPII GLOVER
dc.subjectPIRIMICARB
dc.subjectIDENTIFICATION
dc.subjectPOINT MUTATIONS
dc.subjectTriazophos
dc.subjectINSENSITIVE ACETYLCHOLINESTERASE
dc.subjectInsecticide resistance
dc.subjectAMINO-ACID SUBSTITUTION
dc.subjectINSECTICIDE RESISTANCE
dc.subjectCOTTON APHID
dc.subjectAgricultural and Biological Sciences(all)
dc.titleMutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae)en
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dcterms.dateAccepted2009-01-09
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.bbrc.2008.11.046
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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