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dc.contributor.authorBass, Chris
dc.contributor.authorPuinean, Alin M.
dc.contributor.authorAndrews, Melanie
dc.contributor.authorCutler, Penny
dc.contributor.authorDaniels, Miriam
dc.contributor.authorElias, Jan
dc.contributor.authorPaul, Verity Laura
dc.contributor.authorCrossthwaite, Andrew J.
dc.contributor.authorDenholm, Ian
dc.contributor.authorField, Linda M.
dc.contributor.authorFoster, Stephen P.
dc.contributor.authorLind, Rob
dc.contributor.authorWilliamson, Martin S.
dc.contributor.authorSlater, Russell
dc.date.accessioned2013-09-04T08:30:00Z
dc.date.available2013-09-04T08:30:00Z
dc.date.issued2011-05-31
dc.identifier.citationBass , C , Puinean , A M , Andrews , M , Cutler , P , Daniels , M , Elias , J , Paul , V L , Crossthwaite , A J , Denholm , I , Field , L M , Foster , S P , Lind , R , Williamson , M S & Slater , R 2011 , ' Mutation of a nicotinic acetylcholine receptor beta subunit is associated with resistance to neonicotinoid insecticides in the aphid Myzus persicae ' , BMC Neuroscience , vol. 12 , 51 . https://doi.org/10.1186/1471-2202-12-51
dc.identifier.issn1471-2202
dc.identifier.otherPURE: 1935594
dc.identifier.otherPURE UUID: 7220ce03-102d-4dea-9a71-1fe09d678f2a
dc.identifier.otherWOS: 000291969900001
dc.identifier.otherScopus: 79957661284
dc.identifier.otherORCID: /0000-0001-9797-874X/work/35873691
dc.identifier.urihttp://hdl.handle.net/2299/11521
dc.descriptionThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
dc.description.abstractBackground: Myzus persicae is a globally important aphid pest with a history of developing resistance to insecticides. Unusually, neonicotinoids have remained highly effective as control agents despite nearly two decades of steadily increasing use. In this study, a clone of M. persicae collected from southern France was found, for the first time, to exhibit sufficiently strong resistance to result in loss of the field effectiveness of neonicotinoids. Results: Bioassays, metabolism and gene expression studies implied the presence of two resistance mechanisms in the resistant clone, one based on enhanced detoxification by cytochrome P450 monooxygenases, and another unaffected by a synergist that inhibits detoxifying enzymes. Binding of radiolabeled imidacloprid (a neonicotinoid) to whole body membrane preparations showed that the high affinity [3H]-imidacloprid binding site present in susceptible M. persicae is lost in the resistant clone and the remaining lower affinity site is altered compared to susceptible clones. This confers a significant overall reduction in binding affinity to the neonicotinoid target: the nicotinic acetylcholine receptor (nAChR). Comparison of the nucleotide sequence of six nAChR subunit (Mp alpha 1-5 and Mp beta 1) genes from resistant and susceptible aphid clones revealed a single point mutation in the loop D region of the nAChR beta 1 subunit of the resistant clone, causing an arginine to threonine substitution (R81T). Conclusion: Previous studies have shown that the amino acid at this position within loop D is a key determinant of neonicotinoid binding to nAChRs and this amino acid change confers a vertebrate-like character to the insect nAChR receptor and results in reduced sensitivity to neonicotinoids. The discovery of the mutation at this position and its association with the reduced affinity of the nAChR for imidacloprid is the first example of field-evolved target-site resistance to neonicotinoid insecticides and also provides further validation of exisiting models of neonicotinoid binding and selectivity for insect nAChRs.en
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofBMC Neuroscience
dc.rightsOpen
dc.subjectSITE
dc.subjectPEACH-POTATO APHID
dc.subjectSELECTIVITY
dc.subjectHEMIPTERA
dc.titleMutation of a nicotinic acetylcholine receptor beta subunit is associated with resistance to neonicotinoid insecticides in the aphid Myzus persicaeen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionEcology
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2011-05-31
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1186/1471-2202-12-51
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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