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dc.contributor.authorJones, Christopher M.
dc.contributor.authorDaniels, Miriam
dc.contributor.authorAndrews, Melanie
dc.contributor.authorSlater, Russell
dc.contributor.authorLind, Robert J.
dc.contributor.authorGorman, Kevin
dc.contributor.authorWilliamson, Martin S.
dc.contributor.authorDenholm, Ian
dc.date.accessioned2013-10-28T09:00:51Z
dc.date.available2013-10-28T09:00:51Z
dc.date.issued2011-11
dc.identifier.citationJones , C M , Daniels , M , Andrews , M , Slater , R , Lind , R J , Gorman , K , Williamson , M S & Denholm , I 2011 , ' Age-specific expression of a P450 monooxygenase (CYP6CM1) correlates with neonicotinoid resistance in Bemisia tabaci ' , Pesticide Biochemistry and Physiology , vol. 101 , no. 1 , pp. 53-58 . https://doi.org/10.1016/j.pestbp.2011.07.004
dc.identifier.issn0048-3575
dc.identifier.otherPURE: 1935472
dc.identifier.otherPURE UUID: 2e14cbb0-ae57-402a-b383-6d73e200e981
dc.identifier.otherWOS: 000295152800009
dc.identifier.otherScopus: 80052266280
dc.identifier.otherORCID: /0000-0001-9797-874X/work/35873690
dc.identifier.urihttp://hdl.handle.net/2299/11870
dc.description.abstractThe whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is an invasive insect pest of agriculture and horticulture worldwide. The neonicotinoid insecticides, which target nicotinic acetylcholine receptors in post-synaptic nerve membranes, play a major role in controlling B. tabaci although the increasing incidence of resistance to these compounds is now undermining their effectiveness. Neonicotinoid resistance has been shown to be caused by up-regulation of a cytochrome P450-dependent monooxygenase gene, CYP6CM1, involved in insecticide detoxification. The main aim of this study was to further investigate the finding that expression of resistance to the neonicotinoid imidacloprid is largely restricted to adults of B. tabaci, whereas immature stages retain susceptibility. Imidacloprid resistance was first characterised in four strains of B. tabaci (B- and Q-biotypes) using bioassays, biochemical and molecular-based techniques. A relative gene expression assay was then developed to measure mRNA levels of CYP6CM1 in whitely adults, pupae and nymphs. We show that CYP6CM1 expression correlated with age-specific imidacloprid resistance in adults and nymphs from each strain. Furthermore, in adult whiteflies, the up-regulation of this gene was correlated with imidacloprid metabolism into a hydroxy metabolite as determined by liquid chromatography-mass spectrometry (LC-MS). These findings strengthen the involvement of CYP6CM1 imidacloprid detoxification and suggest that transcriptional changes in CYP6CM1, possibly constrained by the role of P450 enzymes in insect development, are the cause of an age-specific resistance phenotype in B. tabaci. (C) 2011 Elsevier Inc. All rights reserved.en
dc.format.extent6
dc.language.isoeng
dc.relation.ispartofPesticide Biochemistry and Physiology
dc.subjectNILAPARVATA-LUGENS
dc.subjectImidacloprid
dc.subjectMetabolic resistance
dc.subjectANOPHELES-GAMBIAE
dc.subjectMYZUS-PERSICAE
dc.subjectBROWN PLANTHOPPER
dc.subjectMATING INTERACTIONS
dc.subjectOVER-EXPRESSION
dc.subjectCROSS-RESISTANCE
dc.subjectNeonicotinoid
dc.subjectP450 enzyme
dc.subjectOXIDASE-I
dc.subjectBemisia tabaci
dc.subjectB-BIOTYPE
dc.subjectINSECTICIDE RESISTANCE
dc.titleAge-specific expression of a P450 monooxygenase (CYP6CM1) correlates with neonicotinoid resistance in Bemisia tabacien
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.contributor.institutionEcology
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.pestbp.2011.07.004
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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