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dc.contributor.authorVauzour, David
dc.contributor.authorBuonfiglio, Maria
dc.contributor.authorCorona, Giulia
dc.contributor.authorChirafisi, Joselita
dc.contributor.authorVafeiadou, Katerina
dc.contributor.authorAngeloni, Cristina
dc.contributor.authorHrelia, Silvana
dc.contributor.authorHrelia, Patrizia
dc.contributor.authorSpencer, Jeremy P. E.
dc.date.accessioned2013-10-28T12:00:51Z
dc.date.available2013-10-28T12:00:51Z
dc.date.issued2010-04
dc.identifier.citationVauzour , D , Buonfiglio , M , Corona , G , Chirafisi , J , Vafeiadou , K , Angeloni , C , Hrelia , S , Hrelia , P & Spencer , J P E 2010 , ' Sulforaphane protects cortical neurons against 5-S-cysteinyl-dopamine-induced toxicity through the activation of ERK1/2, Nrf-2 and the upregulation of detoxification enzymes ' , Molecular Nutrition and Food Research , vol. 54 , no. 4 , pp. 532-42 . https://doi.org/10.1002/mnfr.200900197
dc.identifier.issn1613-4133
dc.identifier.otherPURE: 2228280
dc.identifier.otherPURE UUID: aebd1d45-be79-4f3b-ac94-babfb9f050eb
dc.identifier.otherPubMed: 20166144
dc.identifier.otherScopus: 77951076084
dc.identifier.urihttp://hdl.handle.net/2299/11879
dc.description.abstractThe degeneration of dopaminergic neurons in the substantia nigra has been linked to the formation of the endogenous neurotoxin 5-S-cysteinyl-dopamine. Sulforaphane (SFN), an isothiocyanate derived from the corresponding precursor glucosinolate found in cruciferous vegetables has been observed to exert a range of biological activities in various cell populations. In this study, we show that SFN protects primary cortical neurons against 5-S-cysteinyl-dopamine induced neuronal injury. Pre-treatment of cortical neurons with SFN (0.01-1 microM) resulted in protection against 5-S-cysteinyl-dopamine-induced neurotoxicity, which peaked at 100 nM. This protection was observed to be mediated by the ability of SFN to modulate the extracellular signal-regulated kinase 1 and 2 and the activation of Kelch-like ECH-associated protein 1/NF-E2-related factor-2 leading to the increased expression and activity of glutathione-S-transferase (M1, M3 and M5), glutathione reductase, thioredoxin reductase and NAD(P)H oxidoreductase 1. These data suggest that SFN stimulates the NF-E2-related factor-2 pathway of antioxidant gene expression in neurons and may protect against neuronal injury relevant to the aetiology of Parkinson's disease.en
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofMolecular Nutrition and Food Research
dc.subjectAnimals
dc.subjectCell Death
dc.subjectCells, Cultured
dc.subjectCerebral Cortex
dc.subjectDopamine
dc.subjectEnzyme Activation
dc.subjectExtracellular Signal-Regulated MAP Kinases
dc.subjectGlutathione
dc.subjectGlutathione Reductase
dc.subjectGlutathione Transferase
dc.subjectMice
dc.subjectMitogen-Activated Protein Kinase 1
dc.subjectMitogen-Activated Protein Kinase 3
dc.subjectNAD(P)H Dehydrogenase (Quinone)
dc.subjectNF-E2-Related Factor 2
dc.subjectNeurons
dc.subjectNeuroprotective Agents
dc.subjectPhosphorylation
dc.subjectThiocyanates
dc.subjectThioredoxin-Disulfide Reductase
dc.titleSulforaphane protects cortical neurons against 5-S-cysteinyl-dopamine-induced toxicity through the activation of ERK1/2, Nrf-2 and the upregulation of detoxification enzymesen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionNutrition and Dietetics
dc.contributor.institutionWeight and Obesity Research Group
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dcterms.dateAccepted2010-04
rioxxterms.versionofrecordhttps://doi.org/10.1002/mnfr.200900197
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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