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dc.contributor.authorKaratolos, Nikos
dc.contributor.authorWilliamson, Martin S.
dc.contributor.authorDenholm, Ian
dc.contributor.authorGorman, Kevin
dc.contributor.authorFfrench-Constant, Richard H.
dc.contributor.authorBass, Chris
dc.date.accessioned2013-12-02T14:30:04Z
dc.date.available2013-12-02T14:30:04Z
dc.date.issued2012-02-08
dc.identifier.citationKaratolos , N , Williamson , M S , Denholm , I , Gorman , K , Ffrench-Constant , R H & Bass , C 2012 , ' Over-Expression of a Cytochrome P450 Is Associated with Resistance to Pyriproxyfen in the Greenhouse Whitefly Trialeurodes vaporariorum ' , PLoS ONE , vol. 7 , no. 2 , e31077 . https://doi.org/10.1371/journal.pone.0031077
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 1935308
dc.identifier.otherPURE UUID: 62398dc2-a56b-40b7-9fb6-2cec8a2beefa
dc.identifier.otherWOS: 000302730100040
dc.identifier.otherScopus: 84856730394
dc.identifier.urihttp://hdl.handle.net/2299/12240
dc.descriptionCopyright: 2012 Karatolos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.description.abstractBackground: The juvenile hormone mimic, pyriproxyfen is a suppressor of insect embryogenesis and development, and is effective at controlling pests such as the greenhouse whitefly Trialeurodes vaporariorum (Westwood) which are resistant to other chemical classes of insecticides. Although there are reports of insects evolving resistance to pyriproxyfen, the underlying resistance mechanism(s) are poorly understood. Results: Bioassays against eggs of a German (TV8) population of T. vaporariorum revealed a moderate level (21-fold) of resistance to pyriproxyfen. This is the first time that pyriproxyfen resistance has been confirmed in this species. Sequential selection of TV8 rapidly generated a strain (TV8pyrsel) displaying a much higher resistance ratio (>4000-fold). The enzyme inhibitor piperonyl butoxide (PBO) suppressed this increased resistance, indicating that it was primarily mediated via metabolic detoxification. Microarray analysis identified a number of significantly over-expressed genes in TV8pyrsel as candidates for a role in resistance including cytochrome-P450 dependent monooxygenases (P450s). Quantitative PCR highlighted a single P450 gene (CYP4G61) that was highly over-expressed (81.7-fold) in TV8pyrsel. Conclusion: Over-expression of a single cytochrome P450 gene (CYP4G61) has emerged as a strong candidate for causing the enhanced resistance phenotype. Further work is needed to confirm the role of the encoded P450 enzyme CYP4G61 in detoxifying pyriproxyfen.en
dc.format.extent8
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.rights/dk/atira/pure/core/openaccesspermission/open
dc.subjectHOMOPTERA
dc.subjectAEDES-AEGYPTI
dc.subjectALEYRODIDAE
dc.subjectHEMIPTERA
dc.subjectINSECT GROWTH-REGULATOR
dc.subjectXENOBIOTICS
dc.subjectBEMISIA-TABACI
dc.subjectMECHANISMS
dc.subjectJUVENILE-HORMONE
dc.subjectDETOXIFICATION GENES
dc.titleOver-Expression of a Cytochrome P450 Is Associated with Resistance to Pyriproxyfen in the Greenhouse Whitefly Trialeurodes vaporariorumen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.contributor.institutionEcology
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1371/journal.pone.0031077
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeopenAccess


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