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dc.contributor.authorGarcia-Nieto, Samuel
dc.contributor.authorJohal, Ramneek K.
dc.contributor.authorShakesheff, Kevin M.
dc.contributor.authorEmara, Mohamed
dc.contributor.authorRoyer, Pierre-Joseph
dc.contributor.authorChau, David Y.S.
dc.contributor.authorShakib, Farouk
dc.contributor.authorGhaemmaghami, Amir M.
dc.date.accessioned2013-12-03T15:29:57Z
dc.date.available2013-12-03T15:29:57Z
dc.date.issued2010-04-19
dc.identifier.citationGarcia-Nieto , S , Johal , R K , Shakesheff , K M , Emara , M , Royer , P-J , Chau , D Y S , Shakib , F & Ghaemmaghami , A M 2010 , ' Laminin and Fibronectin Treatment Leads to Generation of Dendritic Cells with Superior Endocytic Capacity ' , PLoS ONE , vol. 5 , no. 4 , 10123 . https://doi.org/10.1371/journal.pone.0010123
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 2585586
dc.identifier.otherPURE UUID: c2c7fc48-0fb4-455b-bcd5-349c34306a3c
dc.identifier.otherWOS: 000276853800006
dc.identifier.otherScopus: 77956306601
dc.identifier.urihttp://hdl.handle.net/2299/12252
dc.descriptionCopyright: 2010 Garcı´a-Nieto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.description.abstractBackground: Sampling the microenvironment at sites of microbial exposure by dendritic cells ( DC) and their subsequent interaction with T cells in the paracortical area of lymph nodes are key events for initiating immune responses. Most of our knowledge of such events in human is based on in vitro studies performed in the absence of extracellular matrix (ECM) proteins. ECM in basement membranes and interstitial spaces of different tissues, including lymphoid organs, plays an important role in controlling specific cellular functions such as migration, intracellular signalling and differentiation. The aim of this study was, therefore, to investigate the impact of two abundant ECM components, fibronectin and laminin, on the phenotypical and functional properties of DC and how that might influence DC induced T-cell differentiation. Methodology/Principal Findings: Human monocyte derived DC were treated with laminin and fibronectin for up to 48 hours and their morphology and phenotype was analyzed using scanning electron microscopy, flow cytometry and real time PCR. The endocytic ability of DC was determined using flow cytometry. Furthermore, co-culture of DC and T cells were established and T cell proliferation and cytokine profile was measured using H(3)-thymidine incorporation and ELISA respectively. Finally, we assessed formation of DC-T cell conjugates using different cell trackers and flow cytometry. Our data show that in the presence of ECM, DC maintain a 'more immature' phenotype and express higher levels of key endocytic receptors, and as a result become significantly better endocytic cells, but still fully able to mature in response to stimulation as evidenced by their superior ability to induce antigen-specific T cell differentiation. Conclusion: These studies underline the importance of including ECM components in in vitro studies investigating DC biology and DC-T cell interaction. Within the context of antigen specific DC induced T cell proliferation, inclusion of ECM proteins could lead to development of more sensitive assays.en
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.rightsOpen
dc.subjectANTIGEN-PRESENTING CELLS
dc.subjectT-CELLS
dc.subjectLYMPH-NODE
dc.subjectEXTRACELLULAR-MATRIX
dc.subjectPROTEOLYTIC ACTIVITY
dc.subjectMANNOSE RECEPTOR
dc.subjectIN-VIVO
dc.subjectINTEGRINS
dc.subjectPOLARIZATION
dc.subjectINFORMATION
dc.titleLaminin and Fibronectin Treatment Leads to Generation of Dendritic Cells with Superior Endocytic Capacityen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Pharmacy
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2010-04-19
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1371/journal.pone.0010123
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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