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        The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles

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        Author
        Chau, David Y.S.
        Tint, Naing L.
        Collighan, Russell J.
        Griffin, Martin
        Dua, Harminder S.
        Shakesheff, Kevin M.
        Rose, Felicity R. A. J.
        Attention
        2299/12257
        Abstract
        Aims To demonstrate the potential use of in vitro poly (lactic-co-glycolic acid) (PLGA) microparticles in comparison with triamcinolone suspension to aid visualisation of vitreous during anterior and posterior vitrectomy. Methods PLGA microparticles (diameter 10-60 mu m) were fabricated using single and/or double emulsion technique(s) and used untreated or following the surface adsorption of a protein (transglutaminase). Particle size, shape, morphology and surface topography were assessed using scanning electron microscopy (SEM) and compared with a standard triamcinolone suspension. The efficacy of these microparticles to enhance visualisation of vitreous against the triamcinolone suspension was assessed using an in vitro set-up exploiting porcine vitreous. Results Unmodified PLGA microparticles failed to adequately adhere to porcine vitreous and were readily washed out by irrigation. In contrast, modified transglutaminase-coated PLGA microparticles demonstrated a significant improvement in adhesiveness and were comparable to a triamcinolone suspension in their ability to enhance the visualisation of vitreous. This adhesive behaviour also demonstrated selectivity by not binding to the corneal endothelium. Conclusion The use of transglutaminase-modified biodegradable PLGA microparticles represents a novel method of visualising vitreous and aiding vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension.
        Publication date
        2010-05
        Published in
        British Journal of Ophthalmology
        Published version
        https://doi.org/10.1136/bjo.2009.163642
        Other links
        http://hdl.handle.net/2299/12257
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        School of Life and Medical Sciences
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