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        Attachment of stem cells to scaffold particles for intra-cerebral transplantation

        Author
        Bible, Ellen
        Chau, David Y.S.
        Alexander, Morgan R.
        Price, Jack
        Shakesheff, Kevin M.
        Modo, Michel
        Attention
        2299/12324
        Abstract
        Cell-replacement therapy and tissue regeneration using stem cells are of great interest to recover histological damage caused by neuro-degenerative disease or traumatic insults to the brain. To date, the main intra-cerebral delivery for these cells has been as a suspension in media through a thin needle. However, this does not provide cells with a support system that would allow tissue regeneration. Scaffold particles are needed to provide structural support to cells to form de novo tissue. In this 16-d protocol, we describe the generation and functionalization of poly (D,L-lactic-co-glycolic) acid (PLGA) particles to enhance cell attachment, the attachment procedure to avoid clumping and aggregation of cells and particles, and their preparation for intra-cerebral injection through a thin needle. Although the stem cell-scaffold transplantation is more complicated and labor-intensive than cell suspensions, it affords de novo tissue generation inside the brain and hence provides a significant step forward in traumatic brain repair.
        Publication date
        2009
        Published in
        Nature Protocols
        Published version
        https://doi.org/10.1038/nprot.2009.156
        Other links
        http://hdl.handle.net/2299/12324
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