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dc.contributor.authorBible, Ellen
dc.contributor.authorQutachi, Omar
dc.contributor.authorChau, David Y.S.
dc.contributor.authorAlexander, Morgan R.
dc.contributor.authorShakesheff, Kevin M.
dc.contributor.authorModo, Michel
dc.date.accessioned2013-12-19T09:00:13Z
dc.date.available2013-12-19T09:00:13Z
dc.date.issued2012-10
dc.identifier.citationBible , E , Qutachi , O , Chau , D Y S , Alexander , M R , Shakesheff , K M & Modo , M 2012 , ' Neo-vascularization of the stroke cavity by implantation of human neural stem cells on VEGF-releasing PLGA microparticles ' , Biomaterials , vol. 33 , no. 30 , pp. 7435-7446 . https://doi.org/10.1016/j.biomaterials.2012.06.085
dc.identifier.issn0142-9612
dc.identifier.otherPURE: 2585141
dc.identifier.otherPURE UUID: 1ad10340-09b3-4fcd-b290-02f98a176fac
dc.identifier.otherWOS: 000308524000013
dc.identifier.otherScopus: 84864970897
dc.identifier.urihttp://hdl.handle.net/2299/12423
dc.description.abstractReplacing the tissue lost after a stroke potentially provides a new neural substrate to promote recovery. However, significant neurobiological and biotechnological challenges need to be overcome to make this possibility into a reality: Human neural slim cells (hNSCs) can differentiate into mature brain cells, but require a-structural support that retains them within the cavity and affords the formation of a de novo tissue: Nevertheless; in our previous work even after a week, this primitive tissue is void of a vasculature that could sustain its long-term viability. Therefore, tissue engineering strategies are required to develop a vasculature. Vascular endothelial growth factor (VEGF) is known to promote the proliferation and migration of endothelial cells during angio- and arteriogenesis. VEGF by itself here did not affect viability or differentiation of hNSCs, whereas growing cell on poly(D,L-lactic acid-co-glycolic acid) (PLGA) microparticles, with or without VEGF, doubled astrocytic and neuronal differentiation. Secretion of a burst and a sustained delivery of VEGF from the microparticles in vivo attracted endothelial cells from the host into this primitive tissue and in parts established a neovasculature, whereas in other parts endothelial cells were merely interspersed with hNSCs. There was also evidence of a hypervascularization-indicating. that further work will be required to establish an adequate level of vascularization. It is therefore possible to develop a putative neovasculature within de novo tissue that is forming inside a tissue cavity caused by a stroke. (C) 2012 Elsevier Ltd. All rights reserved.en
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofBiomaterials
dc.subjectStroke
dc.subjectNeural stem cells
dc.subjectPLGA
dc.subjectNeo-vascularization
dc.subjectVEGF
dc.subjectAngiogenesis
dc.subjectENDOTHELIAL GROWTH-FACTOR
dc.subjectFOCAL CEREBRAL-ISCHEMIA
dc.subjectBLOOD-BRAIN-BARRIER
dc.subjectADULT-RATS
dc.subjectANGIOGENESIS
dc.subjectNEOVASCULARIZATION
dc.subjectDIFFERENTIATION
dc.subjectPROLIFERATION
dc.subjectMICROSPHERES
dc.subjectBEHAVIOR
dc.titleNeo-vascularization of the stroke cavity by implantation of human neural stem cells on VEGF-releasing PLGA microparticlesen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.biomaterials.2012.06.085
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstyperestrictedAccess


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