Development of a recombinant antibody ELISA test for the detection of Polymyxa betae and its use in resistance screening

Abstract

An ELISA test was developed for the quantitative detection of the obligate parasite Polymyxa betae, the vector of Beet necrotic yellow vein virus (BNYVV), in infected sugarbeet roots. The test used monoclonal and polyclonal antibodies raised to a recombinantly expressed glutathione-S-transferase (GST) from P. betae. A close correlation was found between the number of P. betae zoospores in serially diluted suspensions and absorbance values in the ELISA test. Time-course studies of plants grown in naturally infested soils in controlled environment tests demonstrated the value of the ELISA test in screening for P. betae resistance. In preliminary tests, P. betae-resistant accessions of the wild sea beet (Beta vulgaris ssp. maritima), which might be used to restrict the transmission of BNYVV, were identified