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dc.contributor.authorCalderon, C.
dc.contributor.authorWard, Elaine
dc.contributor.authorFreeman, J.
dc.contributor.authorMcCartney, A.
dc.identifier.citationCalderon , C , Ward , E , Freeman , J & McCartney , A 2002 , ' Detection of airborne fungal spores sampled by rotating-arm and Hirst-type spore traps using polymerase chain reaction assays ' , Journal of Aerosol Science , vol. 33 , no. 2 , pp. 283-296 .
dc.identifier.otherPURE: 1270871
dc.identifier.otherPURE UUID: 7be58612-13c2-477b-b349-5f11356c218b
dc.identifier.otherWOS: 000173318800005
dc.identifier.otherScopus: 0036133106
dc.description.abstractConventional methods for detecting airborne fungal spores rely on either optical identification or culturing and can be time consuming or unreliable. A method for purifying DNA from conventional spore samplers and detecting it using polymerase chain reaction (PCR) assays is described. Experiments were done using Penicillium roqueforti. As few as 10 spores could be detected in the PCR and P. roqueforti spores were detected in a background of spores of six other unrelated species. The method successfully detected P. roqueforti spores collected by rotating arm and Hirst-type spore traps in wind tunnel tests. The tests suggested that the detection limit was about 10 spores or less in the PCR. Fungal spores were also detected in air samples collected in Mexico City using fungal consensus primers, with a detection limit of about 200 spores in the PCR. The potential for using PCR-assays in conjunction with impactor samplers is discussed. (C) 2001 Elsevier Science Ltd. All rights reserved.en
dc.relation.ispartofJournal of Aerosol Science
dc.titleDetection of airborne fungal spores sampled by rotating-arm and Hirst-type spore traps using polymerase chain reaction assaysen
dc.contributor.institutionCrop and Environmental Protection
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
rioxxterms.typeJournal Article/Review

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