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dc.contributor.authorMutasa-Gottgens, Euphemia
dc.contributor.authorChwarszczynska, D.M.
dc.contributor.authorHalsey, K.
dc.contributor.authorAsher , M. J. C.
dc.identifier.citationMutasa-Gottgens , E , Chwarszczynska , D M , Halsey , K & Asher , M J C 2000 , ' Specific polyclonal antibodies for the obligate plant parasite Polymyxa - a targeted recombinant DNA approach ' , Plant Pathology , vol. 49 , no. 2 , pp. 276-287 .
dc.identifier.otherPURE: 1367009
dc.identifier.otherPURE UUID: 0c2683e9-c90e-44e7-a32d-4d95962bcbd2
dc.identifier.otherScopus: 0034097213
dc.description.abstractHighly specific rabbit polyclonal antibodies for the obligate sugar-beet root parasite, Polymyxa betae, were produced using a novel recombinant DNA approach. Parasite cDNA was selectively isolated from infected roots, expressed in vitro, and the purified protein used to raise antibodies. This produced clean, precisely targeted antibodies, and allowed for rigorous screening of candidate genes and their products at the molecular level prior to animal immunization. This approach selects for genes whose products are highly expressed by the parasite in planta, and five such candidate genes from Polymyxa betae were identified and cloned. Polyclonal antiserum developed using the product of one such gene was found to react specifically with P. betae in sugar-beet roots and with the closely related Polymyxa graminis in barley roots, and to cross-react with Plasmodiophora brassicae in cabbage roots, without the need for further purification. No cross-reaction was detected with protein extracts from potato roots infected by the plasmodiophoromycete Spongospora subterranea. In all cases, there was no interaction with proteins from host plants, or from other microorganisms found in association with uninoculated sugar-beet, barley, cabbage and potato rootsen
dc.relation.ispartofPlant Pathology
dc.titleSpecific polyclonal antibodies for the obligate plant parasite Polymyxa - a targeted recombinant DNA approachen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
rioxxterms.typeJournal Article/Review

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