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dc.contributor.authorMorales, F. J.
dc.contributor.authorWard, Elaine
dc.contributor.authorCastano, M.
dc.contributor.authorArroyave, J. A.
dc.contributor.authorLozano, I.
dc.contributor.authorAdams, M. J.
dc.identifier.citationMorales , F J , Ward , E , Castano , M , Arroyave , J A , Lozano , I & Adams , M J 1999 , ' Emergence and partial characterization of rice stripe necrosis virus and its fungus vector in South America ' , European Journal of Plant Pathology , vol. 105 , no. 7 , pp. 643-650 .
dc.identifier.otherPURE: 1295347
dc.identifier.otherPURE UUID: 86fb9af3-e7bd-47f0-93a6-101c7ca99daf
dc.identifier.otherWOS: 000083413800002
dc.identifier.otherScopus: 0032702325
dc.description.abstractA new disease of rice, known as 'entorchamiento' (crinkling), was first noticed in the Department of Meta, Colombia, in 1991. Symptoms include seedling death, foliar striping and severe plant malformation. Tissue extracts and purified preparations from diseased rice plants, contained virus-like particles ca. 20 nm in diameter, with a bimodal length of 260 and 360 nm. Particle aggregates were also observed in the cytoplasm of infected rice leaf cells. Electrophoretic analyses of purified preparations and dsRNA extracts, revealed a single protein species of M-r 22,500, and four dsRNA bands ca. 6300, 4600, 2700 and 1800 bp in size. Cystosori, characteristic of plasmodiophorid fungal vectors of plant viruses, were consistently observed in the roots of diseased rice plants. PCR and sequence analyses of amplified fungal DNA products from infected rice roots, revealed that the putative fungus vector was Polymyxa graminis. A Western blot of tissue extracts obtained from 'crinkled' rice plants from Colombia, using antiserum against a West African isolate of rice stripe necrosis furovirus (RSNV), resulted in the detection of a protein band of approximately M-r 22,000. The RSNV antiserum recognized the Colombian virus isolate in serologically specific electron microscopy tests. These results confirm the presence of RSNV in the Americas.en
dc.relation.ispartofEuropean Journal of Plant Pathology
dc.titleEmergence and partial characterization of rice stripe necrosis virus and its fungus vector in South Americaen
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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