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        Analysis of ribosomal DNA sequences of Polymyxa species and related fungi and the development of genus- and species-specific PCR primers

        Author
        Ward, Elaine
        Adams, M. J.
        Attention
        2299/12621
        Abstract
        A region of about 800 bp of ribosomal DNA has been sequenced in 10 isolates of Polymyxa and one isolate each of Plasmodiophora brassicae and the chytrid Olpidium brassicae. This region consists of about 330 bp at the 3' end of the 18S gene, the 5.8S DNA and the two internal transcribed spacers. A shorter region was also sequenced from one isolate each of Spongospora subterranea and a Ligniera sp. The results supported the earlier division of P. graminis into three subgroups and the recognition of P. betae as a distinct species. In phylogenetic analyses, the Ligniera and Spongospora isolates grouped with the other plasmodiophorids and the Olpidium isolate with the Mycota. The plasmodiophorids appeared as a distinct group and were not closely related to any other eukaryotes (including a range of fungi and protozoa). The DNA sequences were used to design primers for specific amplification of either Polymyxa or P. graminis DNA.
        Publication date
        1998-08
        Published in
        Mycological research
        Published version
        https://doi.org/10.1017/S0953756297005881
        Other links
        http://hdl.handle.net/2299/12621
        Relations
        School of Life and Medical Sciences
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