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dc.contributor.authorBringloe, D.H.
dc.contributor.authorPelpel, M.
dc.contributor.authorCoutts, Robert H.A.
dc.contributor.authorGultyaev, A.P.
dc.contributor.authorPleij, C.W.A.
dc.date.accessioned2014-01-22T15:30:31Z
dc.date.available2014-01-22T15:30:31Z
dc.date.issued1998-06
dc.identifier.citationBringloe , D H , Pelpel , M , Coutts , R H A , Gultyaev , A P & Pleij , C W A 1998 , ' The nucleotide sequence of satellite tobacco necrosis virus strain C and helper-assisted replication of wild-type and mutant clones of the virus ' , Journal of General Virology , vol. 79 , no. 6 , pp. 1539-1546 .
dc.identifier.issn0022-1317
dc.identifier.otherPURE: 1956775
dc.identifier.otherPURE UUID: 83437d63-08c9-4221-bafe-9ade1ff5ff7a
dc.identifier.otherScopus: 0031836986
dc.identifier.urihttp://hdl.handle.net/2299/12622
dc.description.abstractThe complete nucleotide sequence of satellite tobacco necrosis virus strain C (STNV-C) was determined. The genome has a similar overall organization to two STNV isolates studied previously but differs significantly from them in the secondary structure of the translated and untranslated regions (UTRs). STNV-C RNA is naturally uncapped and contains 1221 nt: 101 nt in the 5' UTR, 606 nt in the capsid protein (CP) coding region and 514 nt in the 3' UTR. Using the known sequences of STNV-C and tobacco necrosis virus strain D (TNV-D) RNAs, full-length cDNA clones of both RNAs were constructed. Synthetic transcripts derived from STNV-C cDNA clones only replicated in plants and protoplasts when co-inoculated with TNV-D transcripts. A number of mutant clones in both the 3' and the 5' STNV-C RNA UTRs were constructed which disrupted putative cis-acting elements recognized by helper virus polymerase. Deletion analysis revealed an essential requirement of all 3' and 5' proximal sequences in the STNV-C UTRs for replication. However, an internal region in the 3' UTR could be deleted without loss of infectivity. Likewise, the entire STNV-C CP-encoding region could be deleted and replaced with a marker gene of a similar size without loss of transcript accumulation in plants.en
dc.format.extent8
dc.language.isoeng
dc.relation.ispartofJournal of General Virology
dc.titleThe nucleotide sequence of satellite tobacco necrosis virus strain C and helper-assisted replication of wild-type and mutant clones of the virusen
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionCrop and Environmental Protection
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=0031836986&partnerID=8YFLogxK
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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