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dc.contributor.authorNazir, T.
dc.contributor.authorGould, L. A.
dc.contributor.authorMarriott, C.
dc.contributor.authorMartin, Gary P.
dc.contributor.authorBrown, Marc
dc.date.accessioned2014-01-23T12:00:31Z
dc.date.available2014-01-23T12:00:31Z
dc.date.issued1997-12
dc.identifier.citationNazir , T , Gould , L A , Marriott , C , Martin , G P & Brown , M 1997 , ' High performance liquid chromatography of a cyclosporin A formulation on a porous graphitic carbon column ' , Chromatographia , vol. 46 , no. 11-12 , pp. 628-636 . https://doi.org/10.1007/BF02490523
dc.identifier.issn0009-5893
dc.identifier.otherPURE: 631391
dc.identifier.otherPURE UUID: c629a4fd-d83a-4813-9efb-098d571156b8
dc.identifier.otherWOS: A1997YL62300009
dc.identifier.otherScopus: 0030780173
dc.identifier.urihttp://hdl.handle.net/2299/12640
dc.description.abstractA porous graphitic column (PGC) column was employed as a stationary phase at high temperature to effect the separation of cyclosporin A (CyA) and cyclosporin U (CyU). A robust assay for the former in liposomes was also developed. Linearity was maintained at a concentration range of 2-20 mu g mL(-1) (r(2) = 1.00) with limit of quantification (LOQ) of 200 ng mL(-1). A. %CV (n = 6) of between 0.8-2.0 % showed a good precision of the system. The effects of different temperatures on the peak shape of CyA was also investigated. This showed the process of peak broadening to be kinetically controlled. The study has shown the PGC column to be stable for over 2500 injections which is an improvement over previous assays for CyA analysis.en
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofChromatographia
dc.subjectcolumn liquid chromatography
dc.subjecthigh temperature operation
dc.subjectcyclosporin
dc.subjectconformer
dc.subjectliposomes
dc.subjectWHOLE-BLOOD
dc.subjectHPLC ASSAY
dc.subjectMETABOLITES
dc.subjectEXTRACTION
dc.subjectPLASMA
dc.subjectRAT
dc.subjectPSORIASIS
dc.subjectURINE
dc.subjectFPIA
dc.titleHigh performance liquid chromatography of a cyclosporin A formulation on a porous graphitic carbon columnen
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.contributor.institutionPharmaceutics
dc.contributor.institutionSkin and Nail Group
dc.contributor.institutionAirway Group
dc.contributor.institutionBioadhesive Drug Delivery Group
dc.contributor.institutionNanopharmaceutics
dc.contributor.institutionPharmaceutical Analysis and Product Characterisation
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dcterms.dateAccepted1997-12
rioxxterms.versionofrecordhttps://doi.org/10.1007/BF02490523
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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