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dc.contributor.authorIravani, Mahmoud M.
dc.contributor.authorZar, M. A.
dc.identifier.citationIravani , M M & Zar , M A 1997 , ' The presence and the effects of neuropeptide Y in rat anococcygeus muscle ' , European Journal of Pharmacology , vol. 338 , no. 1 , pp. 75-82 .
dc.identifier.otherPURE: 736141
dc.identifier.otherPURE UUID: 8d410eb9-4316-4bed-895f-7311ee036054
dc.identifier.otherWOS: A1997YE97900011
dc.identifier.otherScopus: 0030695030
dc.identifier.otherORCID: /0000-0002-4905-9682/work/32997606
dc.description.abstractIsolated anococcygeus muscle from male rats was examined for the presence of neuropeptide Y-immunoreactive nerves and for the effects of neuropeptide Y on its tone and its contractile/relaxant responses to electrical field stimulation, acetylcholine, guanethidine and noradrenaline. Using peroxidase anti-peroxidase immunohistochemistry in stretch preparation of the anococcygeus, neuropeptide Y-immunoreactive nerve fibres were observed, in abundance, running along both vascular as well as non-vascular smooth muscle cells. Neuropeptide Y (> 250 nM) evoked phentolamine and tetrodotoxin-resistant contractile response. Neuropeptide Y, even in subspasmogenic concentrations, potentiated contractions evoked by acetylcholine, guanethidine and noradrenaline. Electrical field stimulation (trains of 3-4 pulses, 0.1 ms, 10 Hz) of the isolated anococcygeus preparation produced robust, phentolamine and tetrodotoxin sensitive contractions. Neuropeptide Y (< 10 nM) exerted a biphasic effect on the electrical field stimulation-evoked contractions; an early potentiation was followed by a delayed and progressive inhibition. Neuropeptide Y (> 10 nM) caused a concentration-dependent potentiation of electrical field stimulation-evoked contraction alone, matching its potentiation of noradrenaline-evoked contraction. Electrical field stimulation (5 pulses, 0.1 ms, 10 Hz) of guanethidine (50 mu M)-contracted anococcygeus induced a relaxant response and neuropeptide Y (1-100 nM) exerted a concentration-related slight and variable effect on the electrical field stimulation-evoked relaxant response (1 nM, augmentation; 10 nM, no effect; 100 nM, reduction). It is concluded that rat anococcygeus muscle has a rich neuropeptide Y-containing innervation and neuropeptide Y is mostly stored within adrenergic nerves. The main functional roles of neuropeptide Y in the anococcygeus muscle are likely to be post-junctionally mediated facilitation and prejunctionally mediated inhibition of adrenergic motor transmission. (C) 1997 Elsevier Science B.V.en
dc.relation.ispartofEuropean Journal of Pharmacology
dc.subjectdrenergic transmission
dc.subjectanococcygeus muscle
dc.subjectneuropeptide Y
dc.subjectNANC (non-adrenergic non-cholinergic) transmission
dc.titleThe presence and the effects of neuropeptide Y in rat anococcygeus muscleen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionPharmacology and Clinical Science Research
dc.contributor.institutionNeurodegenerative Diseases
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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