dc.contributor.author | Iravani, Mahmoud M. | |
dc.contributor.author | Zar, M. A. | |
dc.date.accessioned | 2014-01-23T12:00:33Z | |
dc.date.available | 2014-01-23T12:00:33Z | |
dc.date.issued | 1997-10-29 | |
dc.identifier.citation | Iravani , M M & Zar , M A 1997 , ' The presence and the effects of neuropeptide Y in rat anococcygeus muscle ' , European Journal of Pharmacology , vol. 338 , no. 1 , pp. 75-82 . https://doi.org/10.1016/S0014-2999(97)01322-8 | |
dc.identifier.issn | 0014-2999 | |
dc.identifier.other | ORCID: /0000-0002-4905-9682/work/32997606 | |
dc.identifier.uri | http://hdl.handle.net/2299/12642 | |
dc.description.abstract | Isolated anococcygeus muscle from male rats was examined for the presence of neuropeptide Y-immunoreactive nerves and for the effects of neuropeptide Y on its tone and its contractile/relaxant responses to electrical field stimulation, acetylcholine, guanethidine and noradrenaline. Using peroxidase anti-peroxidase immunohistochemistry in stretch preparation of the anococcygeus, neuropeptide Y-immunoreactive nerve fibres were observed, in abundance, running along both vascular as well as non-vascular smooth muscle cells. Neuropeptide Y (> 250 nM) evoked phentolamine and tetrodotoxin-resistant contractile response. Neuropeptide Y, even in subspasmogenic concentrations, potentiated contractions evoked by acetylcholine, guanethidine and noradrenaline. Electrical field stimulation (trains of 3-4 pulses, 0.1 ms, 10 Hz) of the isolated anococcygeus preparation produced robust, phentolamine and tetrodotoxin sensitive contractions. Neuropeptide Y (< 10 nM) exerted a biphasic effect on the electrical field stimulation-evoked contractions; an early potentiation was followed by a delayed and progressive inhibition. Neuropeptide Y (> 10 nM) caused a concentration-dependent potentiation of electrical field stimulation-evoked contraction alone, matching its potentiation of noradrenaline-evoked contraction. Electrical field stimulation (5 pulses, 0.1 ms, 10 Hz) of guanethidine (50 mu M)-contracted anococcygeus induced a relaxant response and neuropeptide Y (1-100 nM) exerted a concentration-related slight and variable effect on the electrical field stimulation-evoked relaxant response (1 nM, augmentation; 10 nM, no effect; 100 nM, reduction). It is concluded that rat anococcygeus muscle has a rich neuropeptide Y-containing innervation and neuropeptide Y is mostly stored within adrenergic nerves. The main functional roles of neuropeptide Y in the anococcygeus muscle are likely to be post-junctionally mediated facilitation and prejunctionally mediated inhibition of adrenergic motor transmission. (C) 1997 Elsevier Science B.V. | en |
dc.format.extent | 8 | |
dc.language.iso | eng | |
dc.relation.ispartof | European Journal of Pharmacology | |
dc.subject | drenergic transmission | |
dc.subject | anococcygeus muscle | |
dc.subject | neuropeptide Y | |
dc.subject | NANC (non-adrenergic non-cholinergic) transmission | |
dc.subject | NANC NERVE-STIMULATION | |
dc.subject | RESPONSES | |
dc.subject | INNERVATION | |
dc.subject | ARGININE | |
dc.title | The presence and the effects of neuropeptide Y in rat anococcygeus muscle | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Centre for Research in Mechanisms of Disease and Drug Discovery | |
dc.contributor.institution | Department of Clinical, Pharmaceutical and Biological Science | |
dc.contributor.institution | Basic and Clinical Science Unit | |
dc.contributor.institution | Centre for Health Services and Clinical Research | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1016/S0014-2999(97)01322-8 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |