dc.contributor.author | Kemp, R. | |
dc.contributor.author | Brennan, R. | |
dc.contributor.author | Lanham, P.G. | |
dc.contributor.author | Slater, R | |
dc.contributor.author | Jones, H. | |
dc.date.accessioned | 2014-01-23T12:30:31Z | |
dc.date.available | 2014-01-23T12:30:31Z | |
dc.date.issued | 1997-04 | |
dc.identifier.citation | Kemp , R , Brennan , R , Lanham , P G , Slater , R & Jones , H 1997 , ' Cloning of cold-inducible dehydrin-like genes from the blackcurrant (Ribes nigrum L.) using RT-PCR ' , Molecular Biotechnology , vol. 7 , no. 2 , pp. 99-102 . https://doi.org/10.1007/BF02761745 | |
dc.identifier.issn | 1559-0305 | |
dc.identifier.other | PURE: 1588855 | |
dc.identifier.other | PURE UUID: 332b3a38-3c0f-459e-a2fe-8cb37fc89a6f | |
dc.identifier.other | Bibtex: urn:e716a7205fc902f84d56c9e440cccbc5 | |
dc.identifier.other | Scopus: 0031109856 | |
dc.identifier.uri | http://hdl.handle.net/2299/12647 | |
dc.description.abstract | A method is described for the identification of conserved genes in one plant species by using sequence information on internal motifs from well-characterized clones from another species. This sequence information is used to design primers for reverse transcriptase polymerase chain reaction (RT-PCR) and to design oligonucleotide probes to identify genuine positive amplification products. The approach was successfully used to clone cDNAs encoding cold-inducible dehydrin-like genes from the woody perennial blackcurrant,Ribes nigrum L. The strategy described can accelerate the cloning of heterologous cDNAs and is a convenient alternative to direct screening of cDNA libraries | en |
dc.format.extent | 4 | |
dc.language.iso | eng | |
dc.relation.ispartof | Molecular Biotechnology | |
dc.title | Cloning of cold-inducible dehydrin-like genes from the blackcurrant (Ribes nigrum L.) using RT-PCR | en |
dc.contributor.institution | Department of Human and Environmental Sciences | |
dc.contributor.institution | Geography, Environment and Agriculture | |
dc.contributor.institution | Microbiology and Biotechnology | |
dc.contributor.institution | Agriculture, Food and Veterinary Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | https://doi.org/10.1007/BF02761745 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |