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dc.contributor.authorKemp, R.
dc.contributor.authorBrennan, R.
dc.contributor.authorLanham, P.G.
dc.contributor.authorSlater, R
dc.contributor.authorJones, H.
dc.identifier.citationKemp , R , Brennan , R , Lanham , P G , Slater , R & Jones , H 1997 , ' Cloning of cold-inducible dehydrin-like genes from the blackcurrant (Ribes nigrum L.) using RT-PCR ' , Molecular Biotechnology , vol. 7 , no. 2 , pp. 99-102 .
dc.identifier.otherPURE: 1588855
dc.identifier.otherPURE UUID: 332b3a38-3c0f-459e-a2fe-8cb37fc89a6f
dc.identifier.otherBibtex: urn:e716a7205fc902f84d56c9e440cccbc5
dc.identifier.otherScopus: 0031109856
dc.description.abstractA method is described for the identification of conserved genes in one plant species by using sequence information on internal motifs from well-characterized clones from another species. This sequence information is used to design primers for reverse transcriptase polymerase chain reaction (RT-PCR) and to design oligonucleotide probes to identify genuine positive amplification products. The approach was successfully used to clone cDNAs encoding cold-inducible dehydrin-like genes from the woody perennial blackcurrant,Ribes nigrum L. The strategy described can accelerate the cloning of heterologous cDNAs and is a convenient alternative to direct screening of cDNA librariesen
dc.relation.ispartofMolecular Biotechnology
dc.titleCloning of cold-inducible dehydrin-like genes from the blackcurrant (Ribes nigrum L.) using RT-PCRen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionMicrobiology and Biotechnology
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionSchool of Life and Medical Sciences
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
rioxxterms.typeJournal Article/Review

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