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dc.contributor.authorRehman, Atif ur
dc.contributor.authorDugic, Elma
dc.contributor.authorBenham, Christopher
dc.contributor.authorLione, Lisa
dc.contributor.authorMacKenzie, Louise Susan
dc.date.accessioned2014-02-05T15:28:57Z
dc.date.available2014-02-05T15:28:57Z
dc.date.issued2014
dc.identifier.citationRehman , A U , Dugic , E , Benham , C , Lione , L & MacKenzie , L S 2014 , ' Selective inhibition of NADPH Oxidase reverses the over contraction of diabetic rat aorta ' , Redox Biology , vol. 2 , pp. 61-64 . https://doi.org/10.1016/j.redox.2013.12.002
dc.identifier.issn2213-2317
dc.identifier.otherPURE: 2583706
dc.identifier.otherPURE UUID: 17ec3d11-f609-4313-9efe-e24da2bc6acf
dc.identifier.otherScopus: 84891455814
dc.identifier.urihttp://hdl.handle.net/2299/12750
dc.descriptionThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use,distribution,and reproduction in any medium, provided the original author and source are credited
dc.description.abstractAbnormal vascular responsiveness in diabetes has been attributed to a number of changes in contractile pathways, affected in part by the overproduction of reactive oxygen species (ROS). It has been reported that NADPH oxidase (NOX) is increased in diabetic (streptozotocin treated; STZ) rat arteries; however the pharmacological agents used to inhibit NOX activity are known to be unsuitable for in vitro studies and have a high level of non-selectivity. Here we have used the highly selective NOX inhibitor VAS2870 in diabetic rat aorta and compared its effects with apocynin, SOD, and allopurinol on phenylephrine and U46619 induced contraction. Male Wistar rats were injected intraperitoneally with 65mg/kg STZ and development of diabetes was confirmed by testing blood glucose levels. Rats were killed by CO2 asphyxiation, and the thoracic aorta removed and mounted in an organ bath under a tension of 1g. Diabetic rat aortas exhibit a greatly increased response to phenylephrine, which was reduced to a level consistent with control rat aorta by 10-5M VAS2870 and 150U/ml SOD .Incubation with VAS2870 led to an increase in normal rat aorta contraction, but led to a significant reduction in phenylephrine and U46619 induced tone in diabetic rat aorta, which indicates that ROS in diabetic rats directly contributes to these contractile responses. Apocynin and allopurinol had no effect on contraction in diabetic or normal rat aorta. This data is the first to show that selective inhibition of NOX reduces diabetic arterial contraction in direct comparison with inhibition of other known contributors of ROS.en
dc.language.isoeng
dc.relation.ispartofRedox Biology
dc.rightsOpen
dc.titleSelective inhibition of NADPH Oxidase reverses the over contraction of diabetic rat aortaen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionPharmacology and Clinical Science Research
dc.contributor.institutionCardiovascular Pathologies
dc.contributor.institutionDiabetic neuropathies
dc.contributor.institutionTRP Ion channels
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2014
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.redox.2013.12.002
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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