Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase
Author
Avila, P.
Delacruz, F.
Ward, Elaine
Grinsted, J.
Attention
2299/12851
Abstract
In the presence of the Tn21 transposase, plasmids that contain a single Tn21 inverted repeat sequence fuse efficiently with other plasmids. This reaction occurs in recA strains, is independent of the transposon-encoded resolution system, and results in insertions into different sites in the recipient plasmid. All fusion products studied contained at least one complete copy of the donor plasmid; most also contained some duplication of it as well. The data are consistent with processive models of transposition