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dc.contributor.authorCoutts, Robert H.A.
dc.contributor.authorWood, K.R.
dc.date.accessioned2014-02-19T15:58:59Z
dc.date.available2014-02-19T15:58:59Z
dc.date.issued1977-05-01
dc.identifier.citationCoutts , R H A & Wood , K R 1977 , ' Improved isolation and culture methods for cucumber mesophyll protoplasts ' , Plant Science Letters , vol. 9 , no. 1 , pp. 45-51 . https://doi.org/10.1016/0304-4211(77)90009-8
dc.identifier.issn0304-4211
dc.identifier.otherPURE: 1957231
dc.identifier.otherPURE UUID: d68c3ca4-c146-448d-a115-a51b0dca17a0
dc.identifier.otherScopus: 0006461517
dc.identifier.urihttp://hdl.handle.net/2299/12863
dc.description.abstractImproved methods for the isolation of large numbers of viable protoplasts from cotyledons and first leaves of two cultivars of cucumber, Cucumis sativus L. cv. Ashley and China are described. Short-term isolation methods (STM), and long-term isolation methods (LTM), using a variety of commercial enzymes are outlined. Liquid culture and subsequent solid culture of isolated first leaf protoplasts resulted in sustained cell division and eventual compact callus formation, which has been induced to form roots. No shoot or whole plant regeneration has, as yet, been demonstrateden
dc.format.extent7
dc.language.isoeng
dc.relation.ispartofPlant Science Letters
dc.titleImproved isolation and culture methods for cucumber mesophyll protoplastsen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=0006461517&partnerID=8YFLogxK
rioxxterms.versionofrecordhttps://doi.org/10.1016/0304-4211(77)90009-8
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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