dc.contributor.author | Sunter, G. | |
dc.contributor.author | Coutts, Robert H.A. | |
dc.contributor.author | Buck, K.W. | |
dc.date.accessioned | 2014-02-27T10:29:04Z | |
dc.date.available | 2014-02-27T10:29:04Z | |
dc.date.issued | 1984-02-14 | |
dc.identifier.citation | Sunter , G , Coutts , R H A & Buck , K W 1984 , ' Negatively supercoiled DNA from plants infected with a single-stranded DNA virus ' , Biochemical and Biophysical Research Communications , vol. 118 , no. 3 , pp. 747-752 . https://doi.org/10.1016/0006-291X(84)91458-X | |
dc.identifier.issn | 0006-291X | |
dc.identifier.uri | http://hdl.handle.net/2299/12936 | |
dc.description.abstract | A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of - 0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme | en |
dc.format.extent | 6 | |
dc.language.iso | eng | |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | |
dc.title | Negatively supercoiled DNA from plants infected with a single-stranded DNA virus | en |
dc.contributor.institution | Agriculture, Food and Veterinary Sciences | |
dc.contributor.institution | Crop Protection and Climate Change | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Department of Clinical, Pharmaceutical and Biological Science | |
dc.contributor.institution | Centre for Agriculture, Food and Environmental Management Research | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=0021762029&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.1016/0006-291X(84)91458-X | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |