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dc.contributor.authorSunter, G.
dc.contributor.authorCoutts, Robert H.A.
dc.contributor.authorBuck, K.W.
dc.date.accessioned2014-02-27T10:29:04Z
dc.date.available2014-02-27T10:29:04Z
dc.date.issued1984-02-14
dc.identifier.citationSunter , G , Coutts , R H A & Buck , K W 1984 , ' Negatively supercoiled DNA from plants infected with a single-stranded DNA virus ' , Biochemical and Biophysical Research Communications , vol. 118 , no. 3 , pp. 747-752 . https://doi.org/10.1016/0006-291X(84)91458-X
dc.identifier.issn0006-291X
dc.identifier.otherPURE: 1957706
dc.identifier.otherPURE UUID: 4465f510-4d31-44dc-83ae-b884afe1f254
dc.identifier.otherScopus: 0021762029
dc.identifier.urihttp://hdl.handle.net/2299/12936
dc.description.abstractA method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of - 0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzymeen
dc.format.extent6
dc.language.isoeng
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.titleNegatively supercoiled DNA from plants infected with a single-stranded DNA virusen
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=0021762029&partnerID=8YFLogxK
rioxxterms.versionofrecordhttps://doi.org/10.1016/0006-291X(84)91458-X
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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