dc.contributor.author | Mutasa-Gottgens, Euphemia | |
dc.contributor.author | Chwarszczynska, D.M. | |
dc.contributor.author | Asher, M. J. C. | |
dc.date.accessioned | 2014-02-27T13:59:00Z | |
dc.date.available | 2014-02-27T13:59:00Z | |
dc.date.issued | 1996-05 | |
dc.identifier.citation | Mutasa-Gottgens , E , Chwarszczynska , D M & Asher , M J C 1996 , ' Single-tube, nested PCR for the diagnosis of Polymyxa betae infection in sugar beet roots and colorimetric analysis of amplified products ' , Phytopathology , vol. 86 , no. 5 , pp. 493-497 . | |
dc.identifier.issn | 0031-949X | |
dc.identifier.uri | http://hdl.handle.net/2299/12952 | |
dc.description.abstract | Nested primers for the specific amplification of DNA sequences from the obligate parasitic root-infecting fungus Polymyxa betae in a single-tube reaction are described. The choice of primers, DNA purity, and relative concentration of outer to inner primers were critical to the success of single-tube reactions. The polymerase chain reaction (PCR) test discriminated against background DNA from the host plant and contaminating microorganisms and detected P. betae in as little as 1 pg of total genomic DNA from infected roots. For rapid analysis of amplified products, primers were modified to generate products that could be detected in a colorimetric assay with the commercially available Captagene-GCN4 kit. It was essential to design a PCR protocol that reduced primer dimerization to levels that did not lead to high background absorbance readings. Results from the Captagene-GCN4 test were compared to those obtained by agarose gel analysis of PCR products. | en |
dc.format.extent | 5 | |
dc.language.iso | eng | |
dc.relation.ispartof | Phytopathology | |
dc.subject | DNA-BINDING PROTEINS | |
dc.subject | rhizomania | |
dc.subject | beet necrotic yellow vein virus | |
dc.subject | plant breeding | |
dc.subject | obligate parasite | |
dc.title | Single-tube, nested PCR for the diagnosis of Polymyxa betae infection in sugar beet roots and colorimetric analysis of amplified products | en |
dc.contributor.institution | Department of Human and Environmental Sciences | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.contributor.institution | Geography, Environment and Agriculture | |
dc.description.status | Peer reviewed | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |