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dc.contributor.authorOffei, S.K.
dc.contributor.authorCoutts, Robert H.A.
dc.identifier.citationOffei , S K & Coutts , R H A 1996 , ' Location of the 5′ termini of tobacco necrosis virus strain D subgenomic mRNAs ' , Journal of Phytopathology , vol. 144 , no. 1 , pp. 13-17 .
dc.identifier.otherPURE: 1955519
dc.identifier.otherPURE UUID: f385dbe0-f390-4d8f-8204-c1d4c138f28a
dc.identifier.otherScopus: 0343060467
dc.description.abstractNorthern blot analysis of double-stranded (ds) RNA from bean-leaf tissue infected with tobacco necrosis virus strain D (TNV-D) detected the 4 kb genomic RNA and two subgenomic RNAs of about 1.5 kb and 1.2 kb; RNA extracted from virus particles only contained the genomic species. Blotting and probing with a range of probes indicated the approximate locations of the 5′ ends of subgenomic RNA so that primers to fine-map the ends could be designed. When both single-stranded and ds RNA, extracted from TNV-D infected and healthy bean leaves were used as templates for primer extension using primers complementary to sequences at, or upstream of, the initiation codons of, respectively, the coat protein and the p7a genes, major infection-specific products were detected. Both subgenomic RNAs start at G residues The larger subgenomic RNA is 1547 nucleotides in length with a leader sequences of 36 nucleotides upstream of the p7a gene, and the smaller subgenomic RNA has a 90 nucleotide leader upstream of the coat protein AUG and is 1202 nucleotides long. An analysis of the 5′ terminal locations of both subgenomic RNAs and the previously mapped analogous subgenomic RNAs associated with infection with the related TNV-A isolate, revealed a marked degree of homology downstream of the initiation sites for each RNA. This homology was maintained at the 5′ termini of both virion RNAs and could be extended to another isolate of TNV for which partial sequence data, but not subgenomic mapping RNA data are available.en
dc.relation.ispartofJournal of Phytopathology
dc.titleLocation of the 5′ termini of tobacco necrosis virus strain D subgenomic mRNAsen
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionCrop and Environmental Protection
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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