dc.contributor.author | Brown, Marc | |
dc.contributor.author | Miller, J. N. | |
dc.contributor.author | Seare, N. J. | |
dc.date.accessioned | 2014-03-04T16:28:59Z | |
dc.date.available | 2014-03-04T16:28:59Z | |
dc.date.issued | 1995-07 | |
dc.identifier.citation | Brown , M , Miller , J N & Seare , N J 1995 , ' An investigation of the use of Nile Red as a long-wavelength fluorescent-probe for the study of alpha(1)-acid glycoprotein-drug interactions ' , Journal of Pharmaceutical and Biomedical Analysis , vol. 13 , no. 8 , pp. 1011-1017 . https://doi.org/10.1016/0731-7085(95)01524-O | |
dc.identifier.issn | 0731-7085 | |
dc.identifier.uri | http://hdl.handle.net/2299/13005 | |
dc.description.abstract | Spectrofluorimetry in the long-wavelength region of the electromagnetic spectrum (600-1000 nm) is a fairly recent development in photoluminescence spectroscopy, which has numerous advantages over measurements in the more conventional ultraviolet and visible spectral region. 9-Diethylamino-5H-benzophenoxazine-5-one (Nile Red) is an unchanged, hydrophobic molecule, and long-wavelength fluorescence of which is strongly influenced by the polarity of its environment. When Nile Red was added to solutions of alpha(1)-acid glycoprotein (Orosomucoid. OMD), it showed an enhancement in fluorescence intensity and a shift to blue in emission wavelength, suggesting it was binding hydrophobically to a non-polar site on the protein. The association constant (12 261 000 +/- 900 000 M(-1)) and number of binding sites (0.746 +/- 0.044) were calculated for the probe. Upon addition of both acidic and basic drugs, the Nile Red fluorescence reverted to its unbound form, indicating that OMD probably has one high-affinity, wide and flexible binding area for such drugs. Possible enantiomeric selectivity was shown with ephedrine, and the association constant determined for a racemic mixture of propranolol was found to be comparable to other values obtained with alternative, more conventional techniques. | en |
dc.format.extent | 7 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of Pharmaceutical and Biomedical Analysis | |
dc.subject | ALPHA(1)-ACID GLYCOPROTEIN | |
dc.subject | DRUG DISPLACEMENT | |
dc.subject | FLUORESCENCE | |
dc.subject | LONG-WAVELENGTH | |
dc.subject | NILE RED | |
dc.subject | HUMAN ALPHA-1-ACID GLYCOPROTEIN | |
dc.subject | ADRENERGIC LIGAND-BINDING | |
dc.subject | PLASMA-PROTEIN BINDING | |
dc.subject | HUMAN-SERUM | |
dc.subject | PROPRANOLOL ENANTIOMERS | |
dc.subject | STEREOSELECTIVE BINDING | |
dc.subject | BASIC DRUGS | |
dc.subject | SITES | |
dc.subject | RECEPTORS | |
dc.subject | ALBUMIN | |
dc.title | An investigation of the use of Nile Red as a long-wavelength fluorescent-probe for the study of alpha(1)-acid glycoprotein-drug interactions | en |
dc.contributor.institution | Department of Pharmacy | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.contributor.institution | Centre for Research into Topical Drug Delivery and Toxicology | |
dc.contributor.institution | Pharmaceutics | |
dc.contributor.institution | Skin and Nail Group | |
dc.contributor.institution | Airway Group | |
dc.contributor.institution | Bioadhesive Drug Delivery Group | |
dc.contributor.institution | Nanopharmaceutics | |
dc.contributor.institution | Pharmaceutical Analysis and Product Characterisation | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1016/0731-7085(95)01524-O | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |