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dc.contributor.authorWard, Elaine
dc.contributor.authorAkrofi, A. Y.
dc.date.accessioned2014-03-10T13:58:56Z
dc.date.available2014-03-10T13:58:56Z
dc.date.issued1994-02
dc.identifier.citationWard , E & Akrofi , A Y 1994 , ' Identification of fungi in the Gaeumannomycesp-Phialophora complex by RFLPS of PCR-amplified ribosomal DNAs ' , Mycological research , vol. 98 , no. 2 , pp. 219-224 . https://doi.org/10.1016/S0953-7562(09)80189-5
dc.identifier.issn0953-7562
dc.identifier.otherPURE: 1295669
dc.identifier.otherPURE UUID: 0982d983-6abd-4da1-856a-7d940b0cd03a
dc.identifier.otherWOS: A1994NA55300012
dc.identifier.otherScopus: 0028045644
dc.identifier.urihttp://hdl.handle.net/2299/13057
dc.description.abstractThe polymerase chain reaction (PCR) was used to amplify ribosomal internal transcribed spacer and 5.8S DNA from isolates of Gaeumannomyces graminis, Phialophora graminicola and other root-infecting fungi, isolated mostly from cereals and grasses. Different restriction enzymes were used to digest these amplified rDNAs to find polymorphisms useful in identification. Most of the enzymes tested were useful for discriminating between G. graminis and P. graminicola, and three enzymes (Dde I, Hae III and Hha I) could be used to distinguish between the varieties of G. graminis (tritici, avenae and graminis). However, a few atypical isolates gave intermediate RFLP patterns. The method was found to discriminate between Gaeumannomyces-Phialophora fungi and other organisms and identify G. graminis var. tritici and P. graminicola on infected wheat roots. The approach is a useful addition to the techniques available for the identification of fungi in the Gaeumannomyces-Phialophora complex.en
dc.format.extent6
dc.language.isoeng
dc.relation.ispartofMycological research
dc.titleIdentification of fungi in the Gaeumannomycesp-Phialophora complex by RFLPS of PCR-amplified ribosomal DNAsen
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1016/S0953-7562(09)80189-5
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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