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        Characterization of a 34 kD protein from potato cyst nematodes, using monoclonal-antibodies with potential for species dignosis

        Author
        Robinson, M. P.
        Butcher, G.
        Curtis, R. H.
        Davies, Keith
        Evans, K.
        Attention
        2299/13083
        Abstract
        Two monoclonal antibodies, which differentially recognise the two species of potato cyst nematodes (PCN), Globodera pallida and G. rostochiensis, are described. They have been shown to have potential for quantification of these two species, recognising proteins of the same molecular weight (34 kD) in both species. Further investigation showed these proteins to have isoelectric points at pH values of 5.7 in G. pallida and 5.9 in G. rostochiensis, in common with the proteins used by Fleming and Marks (1983) to differentiate the species of PCN. They are likely to be structurally very similar, with the same physiological function (and therefore similar concentrations) in the two species. In crossreactivity tests with a wide range of soil nematode species, the antibodies reacted strongly only with species of the genus Globodera, and thereby confirmed their potential as the basis of a quantitative immunoassay likely to be useful in management of PCN populations.
        Publication date
        1993-10
        Published in
        Annals of Applied Biology
        Published version
        https://doi.org/10.1111/j.1744-7348.1993.tb04096.x
        Other links
        http://hdl.handle.net/2299/13083
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