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dc.contributor.authorClapp, J. P.
dc.contributor.authorMcKee, R. A.
dc.contributor.authorAllen-Williams, L.
dc.contributor.authorHopley, J. G.
dc.contributor.authorSlater, R. J.
dc.identifier.citationClapp , J P , McKee , R A , Allen-Williams , L , Hopley , J G & Slater , R J 1993 , ' Genomic subtractive hybridization to isolate species-specific DNA sequences in insects ' , Insect Biochemistry and Molecular Biology , vol. 1 , no. 3 , pp. 133-8 .
dc.identifier.otherPURE: 1576828
dc.identifier.otherPURE UUID: 3c90f169-bce0-4843-80b3-26761f59e738
dc.identifier.otherPubMed: 8269091
dc.identifier.otherScopus: 0027769119
dc.description.abstractSelective enrichment has been used in a number of instances for the isolation of species-specific sequences in prokaryotes. This paper reports the successful application of the technique to insects. Genomic probes were derived to the target species D. funebris and D. simulans. The method involves the biotinylation of non-target 'driver' DNA prepared from the closely related species D. melanogaster and its hybridization to homologous sequences in the target DNA. Hybrid molecules were removed from the reaction by incubation with streptavidin followed by phenol extraction, leaving a preparation enriched for target fragments. All DNA fragments isolated in the D. funebris experiments proved to be specific to that species. Five out of twenty-four fragments screened in the D. simulans experiments were specific when screened with homologous DNA and genomic DNA from its sibling species, D. melanogaster.en
dc.relation.ispartofInsect Biochemistry and Molecular Biology
dc.titleGenomic subtractive hybridization to isolate species-specific DNA sequences in insectsen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionMicrobiology and Biotechnology
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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