dc.contributor.author | Henfrey, R. D. | |
dc.contributor.author | Slater, R. J. | |
dc.date.accessioned | 2014-03-24T14:58:58Z | |
dc.date.available | 2014-03-24T14:58:58Z | |
dc.date.issued | 1988 | |
dc.identifier.citation | Henfrey , R D & Slater , R J 1988 , ' In vitro transcription in plant nuclei ' , Methods in Molecular Biology , vol. 4 , pp. 453-63 . https://doi.org/10.1385/0-89603-127-6:453 | |
dc.identifier.issn | 1064-3745 | |
dc.identifier.uri | http://hdl.handle.net/2299/13156 | |
dc.description.abstract | Isolated plant nuclei can be used for fundamental studies on the transcription apparatus. Total RNA polymerase activity can be measured using plant nuclei, and by using different α-amanitin concentrations in the enzyme assay, the individual RNA polymerase I, II, and in activities can be measured (1-5). The assay procedure involves the incubation of nuclei in the presence of the four substrates for RNA synthesis: ATP, GTP, CTP, and UTP. If one of these precursors is supplied as a radio-labeled molecule, transcription can be detected as incorporation of radioactivity into acid-insoluble material. Following incubation, transcription products are precipitated with TCA, collected and washed on glass fiber filter discs, and counted by liquid scintillation counting. | en |
dc.format.extent | 11 | |
dc.language.iso | eng | |
dc.relation.ispartof | Methods in Molecular Biology | |
dc.title | In vitro transcription in plant nuclei | en |
dc.contributor.institution | Department of Human and Environmental Sciences | |
dc.contributor.institution | Geography, Environment and Agriculture | |
dc.contributor.institution | Microbiology and Biotechnology | |
dc.contributor.institution | Agriculture, Food and Veterinary Sciences | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1385/0-89603-127-6:453 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |