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        The behaviour of tomato golden mosaic virus DNA in cultured cells isolated from systemically infected tobacco leaves

        Author
        Slomka, M.J.
        Buck, K.W.
        Coutts, Robert H.A.
        Attention
        2299/13174
        Abstract
        When callus tissue was cultured from leaf pieces taken from a Nicotiana tabacum cv. Xanthi nc. plant systemically infected with tomato golden mosaic virus (TGMV), TGMV-specific DNA persisted for up to 6 months in culture. Analysis of TGMV-specific intracellular DNA forms indicated a decrease in double-stranded relative to single-stranded forms and an increase in sub-genomic relative to genomic single-stranded DNA species in the callus tissue compared to those in the original leaf explant. The implications of the results with regard to TGMV replication are discussed
        Publication date
        1989-03
        Published in
        Plant Cell Reports
        Published version
        https://doi.org/10.1007/BF00272053
        Other links
        http://hdl.handle.net/2299/13174
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