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dc.contributor.authorCoutts, Robert H.A.
dc.contributor.authorRigden, J.E.
dc.contributor.authorSlabas, A.R.
dc.contributor.authorLomonossoff, G.P.
dc.contributor.authorWise, P.J.
dc.identifier.citationCoutts , R H A , Rigden , J E , Slabas , A R , Lomonossoff , G P & Wise , P J 1991 , ' The complete nucleotide sequence of tobacco necrosis virus strain D ' , Journal of General Virology , vol. 72 , no. 7 , pp. 1521-1529 .
dc.identifier.otherPURE: 1955980
dc.identifier.otherPURE UUID: ee7a41a6-e5c2-40ce-8ee1-48e3d7ebe035
dc.identifier.otherScopus: 0025879484
dc.description.abstractThe complete sequence of the RNA genome of tobacco necrosis virus strain D (TNV-D) consisting of 3759 nucleotides has been determined. The positive strand contains five open reading frames (ORFs). The 5'proximal ORF encodes a 22K protein terminating with an amber codon which may be read through to produce a 82K protein (p82). Two small centrally located ORFs each encode two out-of-frame 7K proteins (p7a and p7b). The 3'-proximal ORF encodes the 29K coat protein (CP), the N terminus of which has been sequenced directly. The genomic organization of TNVD is very similar to that of TNV-A but differs in the placement of the p7a ORF, which does not overlap the p82 ORF in TNV-D, and in the absence of an ORF downstream of the CP gene in TNV-D. The p82 ORF shows extensive sequence similarity with the putative polymerases of the carmovirus group. This ORF is also as closely related to the corresponding ORF of TNV-A as it is to the corresponding ORF of the tombusvirus cucumber necrosis virus. The amino acid sequence of the TNV-D CP gene is similar to both the TNV-A and southern bean mosaic virus CP genes. Of the two p7 ORFs, p7a exhibits amino acid sequence similarity with corresponding proteins from TNV-A, melon necrotic spot virus, carnation mottle virus, turnip crinkle virus and maize chlorotic mottle virus, whereas the p7b ORF appears to be unique to TNV-A and TNV-D. Only the 3'-terminal three nucleotides of TNV-D genomic RNA are identical to the 3'-terminal nucleotides of the TNV satellite virus.en
dc.relation.ispartofJournal of General Virology
dc.titleThe complete nucleotide sequence of tobacco necrosis virus strain Den
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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