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dc.contributor.authorWard, Elaine
dc.identifier.citationWard , E 1995 , ' Improved polymerase chain-reaction (PCR) detection of Gaeumannomyces-graminis including a safeguard against false negatives ' , European Journal of Plant Pathology , vol. 101 , no. 5 , pp. 561-566 .
dc.identifier.otherPURE: 1295647
dc.identifier.otherPURE UUID: 0d173be7-7d82-4fad-ba11-f5b2f3f3d36f
dc.identifier.otherWOS: A1995RT49200012
dc.identifier.otherScopus: 0028813720
dc.description.abstractA previously reported method for polymerase chain reaction (PCR) detection of Gaeumannomyces graminis was modified to simplify it, improve its specificity and decrease the possibility of contamination of the assay. The modified method also allowed discrimination between the G. graminis varieties (tritici and avenae) that are pathogenic to wheat and oats respectively and the variety weakly pathogenic to wheat (var. graminis). An internal positive control for the PCR was also added to the test by including a second pair of primers in the reaction. This positive control has wider application in PCR tests for detection of other fungi.en
dc.relation.ispartofEuropean Journal of Plant Pathology
dc.titleImproved polymerase chain-reaction (PCR) detection of Gaeumannomyces-graminis including a safeguard against false negativesen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.description.statusPeer reviewed

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