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dc.contributor.authorRietveld, A.
dc.contributor.authorBerkhout, Theo
dc.contributor.authorRoenhorst, A.
dc.contributor.authorMarsh, D.
dc.contributor.authorde Kruijff, Ben
dc.date.accessioned2014-06-11T08:30:33Z
dc.date.available2014-06-11T08:30:33Z
dc.date.issued1986-06-13
dc.identifier.citationRietveld , A , Berkhout , T , Roenhorst , A , Marsh , D & de Kruijff , B 1986 , ' Preferential association of apocytochrome c with negatively charged phospholipids in mixed model membranes ' , Biochimica et Biophysica Acta - Biomembranes , vol. 858 , no. 1 , pp. 38-46 . https://doi.org/10.1016/0005-2736(86)90289-0
dc.identifier.issn0006-3002
dc.identifier.otherPURE: 7130937
dc.identifier.otherPURE UUID: 8b1f370b-49cc-4247-8bfb-3f3cdc3fa0bd
dc.identifier.otherPubMed: 3011094
dc.identifier.otherScopus: 0022499342
dc.identifier.urihttp://hdl.handle.net/2299/13685
dc.description.abstractThe mitochondrial precursor protein, apocytochrome c, binds to model membranes containing negatively charged phospholipids (Rietveld, A., Sijens, R., Verkleij, A.J. and Kruijff, B. (1983) EMBO J. 2, 907-913). In the present paper the effect of apocytochrome c on the lipid distribution in model membranes, consisting of neutral and acidic phospholipids, is examined. Both ESR and fluorescence energy transfer experiments show that the protein preferentially interacts with the negatively charged phospholipid in the mixed model membranes. Semi-quantitative analysis of the fluorescence energy transfer from the single tryptophan in apocytochrome c to the parinaric acid in phosphatidylserine or phosphatidylcholine in mixed bovine brain phosphatidylserine/egg phosphatidylcholine vesicles reveals and average donor-acceptor distance of 22-26 A and 26-30 A for phosphatidylserine and phosphatidylcholine, respectively. In addition, these experiments demonstrate that this preferential interaction does not induce the separation of large domains enriched in complexes of apocytochrome c with negatively charged phospholipids and domains enriched in neutral lipids.en
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofBiochimica et Biophysica Acta - Biomembranes
dc.subjectApoproteins
dc.subjectBiological Transport
dc.subjectCytochrome c Group
dc.subjectCytochromes c
dc.subjectElectron Spin Resonance Spectroscopy
dc.subjectEnergy Transfer
dc.subjectMembrane Fluidity
dc.subjectMembrane Lipids
dc.subjectPhosphatidylcholines
dc.subjectPhosphatidylglycerols
dc.subjectPhosphatidylserines
dc.subjectPhospholipids
dc.subjectSpectrometry, Fluorescence
dc.subjectTryptophan
dc.titlePreferential association of apocytochrome c with negatively charged phospholipids in mixed model membranesen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionMedicinal and Analytical Chemistry
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1016/0005-2736(86)90289-0
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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