dc.contributor.author | Berkhout, Theo | |
dc.contributor.author | Gohil, Jayneeta | |
dc.contributor.author | Gonzalez, Pilar | |
dc.contributor.author | Nicols, Charlotte L. | |
dc.contributor.author | Moores, Kitty E. | |
dc.contributor.author | MacPhee, Colin H. | |
dc.contributor.author | White, John R. | |
dc.contributor.author | Groot, Pieter H. E. | |
dc.date.accessioned | 2014-06-11T11:30:36Z | |
dc.date.available | 2014-06-11T11:30:36Z | |
dc.date.issued | 2000-03-01 | |
dc.identifier.citation | Berkhout , T , Gohil , J , Gonzalez , P , Nicols , C L , Moores , K E , MacPhee , C H , White , J R & Groot , P H E 2000 , ' Selective binding of the truncated form of the chemokine CKβ8 (25-99) to CC chemokine receptor 1 (CCR1) ' , Biochemical Pharmacology , vol. 59 , no. 5 , pp. 591-596 . https://doi.org/10.1016/S0006-2952(99)00354-8 | |
dc.identifier.issn | 0006-2952 | |
dc.identifier.other | PURE: 7129825 | |
dc.identifier.other | PURE UUID: e062d086-3112-4107-aef5-752533319ffe | |
dc.identifier.other | Scopus: 0033985115 | |
dc.identifier.other | PubMed: 10660125 | |
dc.identifier.uri | http://hdl.handle.net/2299/13702 | |
dc.description.abstract | Human CC chemokine receptor 1 (CCR1) has been proposed as a receptor for CKβ8. To obtain conclusive evidence, binding-displacement studies of 125I-CKβ8 (25-99) were performed on membranes of Chinese hamster ovary cells expressing human CCR1. The IC50 for displacement of 125I-CKβ8 (25-99) with CKβ8 (25-99) was 0.22 nM. The longer forms of CKβ8 (24-99 and 1-99) also displaced 125I-CKβ8, with IC50 values of 6.5 and 16 nM, respectively. Displacement profiles of 125I-CKβ8 (25-99) on freshly prepared human monocytes indicated that CCR1 was the major receptor for CKβ8. We conclude that CCR1 is a receptor for different-length CKβ8 and that CKβ8 (25-99) has a similar affinity for CCR1 as macrophage inflammatory protein-1α (MIP-1α). The longer variants of CKβ8 are significantly less potent than CKβ8 (25-99) and MIP-1α on CCR1 and monocytes (P < 0.05). Copyright (C) 2000 Elsevier Science Inc. | en |
dc.format.extent | 6 | |
dc.language.iso | eng | |
dc.relation.ispartof | Biochemical Pharmacology | |
dc.subject | Chemokine | |
dc.subject | Chemokine receptor | |
dc.subject | CKβ8 | |
dc.subject | Human CC chemokine receptor 1 (CCR1) | |
dc.subject | Ligand binding | |
dc.subject | Monocytes | |
dc.subject | Pharmacology | |
dc.title | Selective binding of the truncated form of the chemokine CKβ8 (25-99) to CC chemokine receptor 1 (CCR1) | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Department of Pharmacy | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.contributor.institution | Medicinal and Analytical Chemistry | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | https://doi.org/10.1016/S0006-2952(99)00354-8 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |