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dc.contributor.authorBerkhout, Theo
dc.contributor.authorGohil, Jayneeta
dc.contributor.authorGonzalez, Pilar
dc.contributor.authorNicols, Charlotte L.
dc.contributor.authorMoores, Kitty E.
dc.contributor.authorMacPhee, Colin H.
dc.contributor.authorWhite, John R.
dc.contributor.authorGroot, Pieter H. E.
dc.identifier.citationBerkhout , T , Gohil , J , Gonzalez , P , Nicols , C L , Moores , K E , MacPhee , C H , White , J R & Groot , P H E 2000 , ' Selective binding of the truncated form of the chemokine CKβ8 (25-99) to CC chemokine receptor 1 (CCR1) ' , Biochemical Pharmacology , vol. 59 , no. 5 , pp. 591-596 .
dc.identifier.otherPURE: 7129825
dc.identifier.otherPURE UUID: e062d086-3112-4107-aef5-752533319ffe
dc.identifier.otherScopus: 0033985115
dc.identifier.otherPubMed: 10660125
dc.description.abstractHuman CC chemokine receptor 1 (CCR1) has been proposed as a receptor for CKβ8. To obtain conclusive evidence, binding-displacement studies of 125I-CKβ8 (25-99) were performed on membranes of Chinese hamster ovary cells expressing human CCR1. The IC50 for displacement of 125I-CKβ8 (25-99) with CKβ8 (25-99) was 0.22 nM. The longer forms of CKβ8 (24-99 and 1-99) also displaced 125I-CKβ8, with IC50 values of 6.5 and 16 nM, respectively. Displacement profiles of 125I-CKβ8 (25-99) on freshly prepared human monocytes indicated that CCR1 was the major receptor for CKβ8. We conclude that CCR1 is a receptor for different-length CKβ8 and that CKβ8 (25-99) has a similar affinity for CCR1 as macrophage inflammatory protein-1α (MIP-1α). The longer variants of CKβ8 are significantly less potent than CKβ8 (25-99) and MIP-1α on CCR1 and monocytes (P < 0.05). Copyright (C) 2000 Elsevier Science Inc.en
dc.relation.ispartofBiochemical Pharmacology
dc.subjectChemokine receptor
dc.subjectHuman CC chemokine receptor 1 (CCR1)
dc.subjectLigand binding
dc.titleSelective binding of the truncated form of the chemokine CKβ8 (25-99) to CC chemokine receptor 1 (CCR1)en
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionMedicinal and Analytical Chemistry
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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