Bioluminescence-based measurement of viability of Pseudomonas aeruginosa ATCC 9027 harbouring plasmid-based lux genes under the control of constitutive promoters

Abstract

Detection of microbial contamination in pharmaceuticals, food and cosmetics has been problematic for several decades. Numerous investigations highlight the urgency for novel methods; development of bioluminescent constructs allows real-time monitoring, rapid analysis and high-throughput screening of products. Microbial growth can be studied by measuring constitutive gene expression. The aim is to develop whole-cell microbial biosensors with Pseudomonas aeruginosa and quantify their growth rate by measuring constitutive expression of lux