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        Bioluminescence-based measurement of viability of Pseudomonas aeruginosa ATCC 9027 harbouring plasmid-based lux genes under the control of constitutive promoters

        Author
        Shah, Niksha
        Naseby, David
        Attention
        2299/14683
        Abstract
        Detection of microbial contamination in pharmaceuticals, food and cosmetics has been problematic for several decades. Numerous investigations highlight the urgency for novel methods; development of bioluminescent constructs allows real-time monitoring, rapid analysis and high-throughput screening of products. Microbial growth can be studied by measuring constitutive gene expression. The aim is to develop whole-cell microbial biosensors with Pseudomonas aeruginosa and quantify their growth rate by measuring constitutive expression of lux
        Publication date
        2014-11
        Published in
        Journal of Applied Microbiology
        Published version
        https://doi.org/10.1111/jam.12635
        Other links
        http://hdl.handle.net/2299/14683
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