University of Hertfordshire Research Archive

        JavaScript is disabled for your browser. Some features of this site may not work without it.

        Browse

        All of UHRABy Issue DateAuthorsTitlesThis CollectionBy Issue DateAuthorsTitles

        Arkivum Files

        My Downloads
        View Item 
        • UHRA Home
        • University of Hertfordshire
        • Research publications
        • View Item
        • UHRA Home
        • University of Hertfordshire
        • Research publications
        • View Item

        P-419: Phospholipase C isoforms and differentiation state of cultured human Vascular Smooth Muscle Cells.

        Author
        MacKenzie, Louise Susan
        Shelley, C.
        Patel, M.K.
        Hughes, A.D.
        Lymn, Joanne
        Attention
        2299/15161
        Abstract
        Phospholipase C (PLC) plays an important role in cellular signalling. A number of isoforms of PLC exist; PLCγ1 is highly expressed in fetal and neoplastic tissue, while PLCδ1 is present in large amounts in differentiated smooth muscle. The objective of these studies was to examine the effect of conditions affecting differentiation state on patterns of PLC isoform expression in human vascular smooth muscle cells. Human saphenous vein derived smooth muscle cells (HVSMC) were cultured in DMEM containing 15% foetal calf serum. Smooth muscle α-actin (SM α-actin) and PLC isoforms were determined in cell lysates derived from HVSMC (passage 3) by SDS PAGE and Western blotting. Only PLCδ1 and PLCγ1 were detectable in HVSMC, although PLC β1, β3, β4, δ1, δ2, γ1, γ2 were demonstrable in lysates from rat brain. The effect of changing differentiation state on the relative expression of PLCδ1 and PLCγ1 was investigated by serum withdrawal for seven days (SFM), exposure to retinoic acid (10μM) for 72h or exposure to a HMG CoA reductase inhibitor, lovastatin (3μM) for 24h. Western blots were analysed by densitometry. Data are mean±SEM of the % change in protein estimated by densitometry with respect to untreated control HVSMC
        Publication date
        2001
        Published in
        American Journal of Hypertension
        Published version
        https://doi.org/10.1016/S0895-7061(01)01577-1
        Other links
        http://hdl.handle.net/2299/15161
        Metadata
        Show full item record
        Keep in touch

        © 2019 University of Hertfordshire

        I want to...

        • Apply for a course
        • Download a Prospectus
        • Find a job at the University
        • Make a complaint
        • Contact the Press Office

        Go to...

        • Accommodation booking
        • Your student record
        • Bayfordbury
        • KASPAR
        • UH Arts

        The small print

        • Terms of use
        • Privacy and cookies
        • Criminal Finances Act 2017
        • Modern Slavery Act 2015
        • Sitemap

        Find/Contact us

        • T: +44 (0)1707 284000
        • E: ask@herts.ac.uk
        • Where to find us
        • Parking
        • hr
        • qaa
        • stonewall
        • AMBA
        • ECU Race Charter
        • disability confident
        • AthenaSwan