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dc.contributor.authorZhao, Jingxia
dc.contributor.authorYao, Yang
dc.contributor.authorLiu, Shichang
dc.contributor.authorZhang, Tao
dc.contributor.authorRen, Guogang
dc.contributor.authorYang, Zhuo
dc.date.accessioned2015-01-29T10:17:56Z
dc.date.available2015-01-29T10:17:56Z
dc.date.issued2012-11
dc.identifier.citationZhao , J , Yao , Y , Liu , S , Zhang , T , Ren , G & Yang , Z 2012 , ' Involvement of reactive oxygen species and high-voltage-activated calcium currents in nanoparticle zinc oxide-induced cytotoxicity in vitro ' , Journal of Nanoparticle Research , vol. 14 , no. 11 , 1238 . https://doi.org/10.1007/s11051-012-1238-1
dc.identifier.issn1388-0764
dc.identifier.otherPURE: 8017137
dc.identifier.otherPURE UUID: 8191569f-47ea-4872-8c2f-a5476b1a7868
dc.identifier.otherWOS: 000310608100024
dc.identifier.otherScopus: 84867512977
dc.identifier.otherORCID: /0000-0001-8865-1526/work/32373259
dc.identifier.urihttp://hdl.handle.net/2299/15312
dc.description.abstractThis study was to determine the possible neurotoxicity and mechanisms underlying the effects of nano-ZnO with sizes of 20-80 nm on central nervous system (CNS). The cytotoxicity of nano-ZnO was investigated in PC12 cells. The viability of cells was observed by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the generation of reactive oxygen species (ROS) for cells was evaluated by a fluorometry assay. The apoptosis of cells was detected and analyzed by flow cytometry. In addition, effects of nano-ZnO on the properties of high-voltage-activated (HVA) calcium currents were studied in acutely isolated rat hippocampal pyramidal neurons using the whole-cell patch clamp technique. The results of MTT assay showed that nano-ZnO (10(-4) g/mL) caused a significant decrease in cell viability (P <0.05). Nano-ZnO induced intracellular accumulation of ROS and the apoptosis of PC12 cells with the increasing concentration of nano-ZnO in flow cytometric assay (P <0.05). Further results of electrophysiological recording indicated that 10(-4) g/mL nano-ZnO first altered the current-voltage curve and the peak amplitudes of HVA calcium currents at 10 min of the recording, and the peak current amplitudes were increased significantly at the end of 30 min (P <0.05). All these results suggested that the increase of intracellular ROS was one of potential mechanisms of cellular apoptosis induced by nano-ZnO. Nano-ZnO could cause the elevation of cytosolic calcium levels by enhancement of HVA calcium currents, which would increase the generation of intracellular ROS, and consequently promote the neuronal apoptosis.en
dc.format.extent14
dc.language.isoeng
dc.relation.ispartofJournal of Nanoparticle Research
dc.subjectZinc oxide nanoparticles (nano-ZnO)
dc.subjectReactive oxygen species (ROS)
dc.subjectHigh-voltage-activated (HVA) calcium currents
dc.subjectApoptosis
dc.subjectCENTRAL-NERVOUS-SYSTEM
dc.subjectCA2+ CHANNELS
dc.subjectANTIBACTERIAL ACTIVITY
dc.subjectTOXICOLOGICAL IMPACT
dc.subjectCOMPARATIVE TOXICITY
dc.subjectPARTICLE SOLUBILITY
dc.subjectTIO2 NANOPARTICLES
dc.subjectDAPHNIA-MAGNA
dc.subjectBULK ZNO
dc.subjectNEURONS
dc.titleInvolvement of reactive oxygen species and high-voltage-activated calcium currents in nanoparticle zinc oxide-induced cytotoxicity in vitroen
dc.contributor.institutionSchool of Engineering and Technology
dc.contributor.institutionScience & Technology Research Institute
dc.contributor.institutionCentre for Engineering Research
dc.contributor.institutionMaterials and Structures
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1007/s11051-012-1238-1
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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