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dc.contributor.authorShah, Niksha
dc.contributor.authorNaseby, David
dc.identifier.citationShah , N & Naseby , D 2015 , ' Validation of constitutively expressed bioluminescent Pseudomonas aeruginosa as a rapid microbiological quantification tool ' , Biosensors & Bioelectronics , vol. 68 , pp. 447-453 .
dc.identifier.otherPURE: 8148013
dc.identifier.otherPURE UUID: 347407cb-c3d2-4ff1-85d1-868b58b13c63
dc.identifier.otherScopus: 84921491126
dc.descriptionDate of Acceptance: 02/01/2015
dc.description.abstractWhole cell biosensors have been extensively used for monitoring toxicity and contamination of various compounds and xenobiotics in environmental biology and microbial ecology; their application in the pharmaceutical and cosmetics industries has been limited. According to several pharmacopoeias, pharmaceutical products must be tested for microbial activity using traditional viable count techniques; the use of whole cell microbial biosensors potentially provides an alternative, fast, and efficient method. However there is a lack of a validated bioluminescence method. Prototype whole cell microbial biosensors have already been developed in Pseudomonas aeruginosa ATCC 9027. Validation of the bioluminescent strains was performed in accordance with the pharmacopoeia, Parenteral Drug Association and International Organisation of Standardisation. These strains demonstrated that the bioluminescent method was accurate, precise and equivalent, as compared with plate counting at a range of 103–107 CFU/mL. Percentage recoveries using the bioluminescent method were between 70% and 130% for all bioluminescent strains and therefore the bioluminescent method was accurate according to the criteria set in PDA technical report 33. The method was also more precise (relative standard deviation less than 15%) than the traditional plate counting method or the ATP bioluminescent method. The lower limit of detection was 103 CFU/mL. Two-way ANOVA showed no significant difference between the traditional plate counting and the novel bioluminescent method for all bioluminescent strains. The bioluminescent constructs passed/exceeded pharmacopoeia-specified criteria for range, limit of detection, accuracy, precision and equivalenceen
dc.relation.ispartofBiosensors & Bioelectronics
dc.titleValidation of constitutively expressed bioluminescent Pseudomonas aeruginosa as a rapid microbiological quantification toolen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionMicrobiology and Biotechnology
dc.contributor.institutionSchool of Engineering and Technology
dc.contributor.institutionScience & Technology Research Institute
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.relation.schoolSchool of Engineering and Technology
rioxxterms.typeJournal Article/Review

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