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dc.contributor.authorEdman, K.A.P.
dc.contributor.authorLou, Fang
dc.date.accessioned2015-03-04T16:03:29Z
dc.date.available2015-03-04T16:03:29Z
dc.date.issued1990-05
dc.identifier.citationEdman , K A P & Lou , F 1990 , ' Changes in force and stiffness induced by fatigue and intracellular acidification in frog muscle fibres ' , Journal of Physiology , vol. 424 , no. 1 , pp. 133-49 . https://doi.org/10.1113/jphysiol.1990.sp018059
dc.identifier.issn0022-3751
dc.identifier.otherPURE: 8196094
dc.identifier.otherPURE UUID: 4b8778dc-ee36-4150-a259-b45f1d79d1d3
dc.identifier.otherPubMed: 2391650
dc.identifier.otherScopus: 0025281671
dc.identifier.urihttp://hdl.handle.net/2299/15531
dc.description.abstract1. Changes in force and stiffness were recorded simultaneously during 1 s isometric (fixed ends) tetani of single fibres isolated from the anterior tibialis muscle of Rana temporaria (temperature 1-3 degrees C; sarcomere length, 2.10 micron). Stiffness was measured as the change in force that occurred in response to a 4 kHz sinusoidal length oscillation of the fibre. Some experiments were performed in which stiffness was determined from a fast (0.2 ms) length step that was applied to a 'tendon-free' segment of the muscle fibre during the tetanus plateau. 2. A moderate degree of fatigue was produced by decreasing the time between tetani from 300 s (control) to 15 s. By this treatment the maximum tetanic force (Ftet) was reversibly reduced to 70-75% of the control value. Maximum tetanic stiffness (Stet) was related to Ftet according to the following regression (both variables expressed as percentage of their control values): Stet = 0.369 Ftet + 62.91 (correlation coefficient, 0.95; P less than 0.001). A 25% decrease in isometric force during fatigue was thus associated with merely 9% reduction of fibre stiffness. 3. Whereas the rate of rise of force during tetanus was markedly reduced by fatiguing stimulation, the rate of rise of stiffness was only slightly affected. 4. Intracellular acidification (produced by raised extracellular CO2 concentration) largely reproduced the contractile changes observed during fatigue. However, for a given decrease in tetanic force there was a smaller reduction in fibre stiffness during acidosis than during fatigue. 5. Caffeine (0.5 mM) added to the fibre after development of fatigue and intracellular acidosis greatly potentiated the isometric twitch but did not affect maximum tetanic force. This finding provides evidence that the contractile system was fully activated during the tetanus plateau both in the fatigued state and during acidosis. 6. The results suggest that the decrease in contractile strength after frequent tetanization (intervals between tetani, 15 s) is attributable to altered kinetics of cross-bridge function leading to reduced number of active cross-bridges and, most significantly, to reduced force output of the individual bridge. The possible role of increased intracellular H+ concentration in the development of muscle fatigue is discussed.en
dc.format.extent17
dc.language.isoeng
dc.relation.ispartofJournal of Physiology
dc.subjectAnimals
dc.subjectCaffeine
dc.subjectHydrogen-Ion Concentration
dc.subjectIsometric Contraction
dc.subjectMuscles
dc.subjectRana temporaria
dc.titleChanges in force and stiffness induced by fatigue and intracellular acidification in frog muscle fibresen
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionRegistry
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionPharmacology and Clinical Science Research
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1113/jphysiol.1990.sp018059
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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