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dc.contributor.authorSun, Y-B.
dc.contributor.authorLou, Fang
dc.contributor.authorEdman, K.A.P.
dc.date.accessioned2015-03-05T08:48:35Z
dc.date.available2015-03-05T08:48:35Z
dc.date.issued1996-09
dc.identifier.citationSun , Y-B , Lou , F & Edman , K A P 1996 , ' The relationship between the intracellular Ca2+ transient and the isometric twitch force in frog muscle fibres ' , Experimental Physiology , vol. 81 , no. 5 , pp. 711-24 . https://doi.org/10.1113/expphysiol.1996.sp003971
dc.identifier.issn0958-0670
dc.identifier.otherPURE: 8195911
dc.identifier.otherPURE UUID: 472cbdd5-5d5b-4182-87fc-a43efbc84e60
dc.identifier.otherPubMed: 8889472
dc.identifier.otherScopus: 0029658403
dc.identifier.urihttp://hdl.handle.net/2299/15536
dc.description.abstractThe calcium-sensitive fluorescent indicator fluo-3 was used to monitor the intracellular free calcium concentration ([Ca2+]i) during isometric twitches in twenty-nine single muscle fibres from the anterior tibialis muscle of Rana temporaria (sarcomere length, 2.2 microns; 2-4 degrees C). The transient change in [Ca2+]i in response to a single stimulus was very brief. The time to peak and the duration of the Ca2+ signal, measured at 50% of the peak amplitude, were 8.3 +/- 0.2 and 22.1 +/- 1.4 ms (mean +/- S.E.M., n = 29), respectively. The mean peak amplitude of the Ca2+ transient was 3.2 +/- 0.1 microM, ranging from 2.46 to 3.92 microM among the different fibres. The isometric force started to rise 2.5 ms before [Ca2+]i reached its maximum value. When peak twitch force was attained, [Ca2+]i had already declined to approximately 10% of its maximum value. The peak force produced during a twitch was closely related to the decay phase of the Ca2+ transient, a slower decay of [Ca2+]i being associated with a greater amplitude of the twitch. The amplitude and duration of the Ca2+ transient varied in a systematic way relative to one another in different fibres, in that a greater amplitude was associated with a more rapid decay of the Ca2+ transient. NO3- and Zn2+ added to the external medium greatly enhanced the peak twitch force without markedly affecting the amplitude of the Ca2+ transient. However, both agents delayed the decay of [Ca2+]i. It is concluded that the decay phase of the Ca2+ transient is a more important determinant of the mechanical response during an isometric twitch than is the peak amplitude of the transient.en
dc.format.extent14
dc.language.isoeng
dc.relation.ispartofExperimental Physiology
dc.subjectAniline Compounds
dc.subjectAnimals
dc.subjectCalcium
dc.subjectFluorescent Dyes
dc.subjectKinetics
dc.subjectMuscle Contraction
dc.subjectRana temporaria
dc.subjectXanthenes
dc.titleThe relationship between the intracellular Ca2+ transient and the isometric twitch force in frog muscle fibresen
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionRegistry
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionPharmacology and Clinical Science Research
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1113/expphysiol.1996.sp003971
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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