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dc.contributor.authorAwad, Jasmin
dc.contributor.authorStotz, Henrik
dc.contributor.authorFekete, Agnes
dc.contributor.authorKrischke, Markus
dc.contributor.authorEngert, Cornelia
dc.contributor.authorHavaux, Michel
dc.contributor.authorBerger, Susanne
dc.contributor.authorMueller, Martin J.
dc.identifier.citationAwad , J , Stotz , H , Fekete , A , Krischke , M , Engert , C , Havaux , M , Berger , S & Mueller , M J 2015 , ' 2-Cysteine Peroxiredoxins and Thylakoid Ascorbate Peroxidase create a water-water cycle that is essential to protect the photosynthetic apparatus under high light stress conditions ' , Plant Physiology , vol. 167 , no. 4 , pp. 1592-603 .
dc.identifier.otherPURE: 8442250
dc.identifier.otherPURE UUID: 8b5996b0-58bb-4a7b-9447-0d94c7d9bde4
dc.identifier.otherPubMed: 25667319
dc.identifier.otherScopus: 84926218561
dc.descriptionJasmin Awad, Henrik U. Stotz, Agenes Fekete, Markus Krischke, Cornelia Engert, Michel Havaux, Susanne Berger and Martin J. Mueller, ‘2-Cysteine Peroxiredoxins and Thylakoid Ascorbate Peroxidase Create a Water-Water Cycle That Is Essential to Protect the Photosynthetic Apparatus under High Light Stress Conditions’, Plant Physiology, Vol. 167(4): 1592-1603, first published online February 9, 2015, is available online at doi:
dc.description.abstractDifferent peroxidases, including 2-cysteine (2-Cys) peroxiredoxins (PRXs) and thylakoid ascorbate peroxidase (tAPX), have been proposed to be involved in the water-water cycle (WWC) and hydrogen peroxide (H2O2)-mediated signaling in plastids. We generated an Arabidopsis (Arabidopsis thaliana) double-mutant line deficient in the two plastid 2-Cys PRXs (2-Cys PRX A and B, 2cpa 2cpb) and a triple mutant deficient in 2-Cys PRXs and tAPX (2cpa 2cpb tapx). In contrast to wild-type and tapx single-knockout plants, 2cpa 2cpb double-knockout plants showed an impairment of photosynthetic efficiency and became photobleached under high light (HL) growth conditions. In addition, double-mutant plants also generated elevated levels of superoxide anion radicals, H2O2, and carbonylated proteins but lacked anthocyanin accumulation under HL stress conditions. Under HL conditions, 2-Cys PRXs seem to be essential in maintaining the WWC, whereas tAPX is dispensable. By comparison, this HL-sensitive phenotype was more severe in 2cpa 2cpb tapx triple-mutant plants, indicating that tAPX partially compensates for the loss of functional 2-Cys PRXs by mutation or inactivation by overoxidation. In response to HL, H2O2- and photooxidative stress-responsive marker genes were found to be dramatically up-regulated in 2cpa 2cpb tapx but not 2cpa 2cpb mutant plants, suggesting that HL-induced plastid to nucleus retrograde photooxidative stress signaling takes place after loss or inactivation of the WWC enzymes 2-Cys PRX A, 2-Cys PRX B, and tAPX.en
dc.relation.ispartofPlant Physiology
dc.title2-Cysteine Peroxiredoxins and Thylakoid Ascorbate Peroxidase create a water-water cycle that is essential to protect the photosynthetic apparatus under high light stress conditionsen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop Protection and Climate Change
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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