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dc.contributor.authorCook, Michael T.
dc.contributor.authorSaratoon, Teedah
dc.contributor.authorTzortzis, George
dc.contributor.authorEdwards, Alexander
dc.contributor.authorCharalampopoulos, Dimitris
dc.contributor.authorKhutoryanskiy, Vitaliy V.
dc.date.accessioned2015-06-01T14:06:01Z
dc.date.available2015-06-01T14:06:01Z
dc.date.issued2013-02-11
dc.identifier.citationCook , M T , Saratoon , T , Tzortzis , G , Edwards , A , Charalampopoulos , D & Khutoryanskiy , V V 2013 , ' CLSM method for the dynamic observation of pH change within polymer matrices for oral delivery ' , Biomacromolecules , vol. 14 , no. 2 , pp. 387-393 . https://doi.org/10.1021/bm301569r
dc.identifier.issn1525-7797
dc.identifier.otherPURE: 8599488
dc.identifier.otherPURE UUID: 8bce113a-802a-4e4c-aeed-bc72493071fe
dc.identifier.otherScopus: 84873631986
dc.identifier.otherPubMed: 23301617
dc.identifier.urihttp://hdl.handle.net/2299/15969
dc.descriptionDate of Acceptance: 09/01/2013
dc.description.abstractIf acid-sensitive drugs or cells are administered orally, there is often a reduction in efficacy associated with gastric passage. Formulation into a polymer matrix is a potential method to improve their stability. The visualization of pH within these materials may help better understand the action of these polymer systems and allow comparison of different formulations. We herein describe the development of a novel confocal laser-scanning microscopy (CLSM) method for visualizing pH changes within polymer matrices and demonstrate its applicability to an enteric formulation based on chitosan-coated alginate gels. The system in question is first shown to protect an acid-sensitive bacterial strain to low pH, before being studied by our technique. Prior to this study, it has been claimed that protection by these materials is a result of buffering, but this has not been demonstrated. The visualization of pH within these matrices during exposure to a pH 2.0 simulated gastric solution showed an encroachment of acid from the periphery of the capsule, and a persistence of pHs above 2.0 within the matrix. This implies that the protective effect of the alginate-chitosan matrices is most likely due to a combination of buffering of acid as it enters the polymer matrix and the slowing of acid penetration.en
dc.format.extent7
dc.language.isoeng
dc.relation.ispartofBiomacromolecules
dc.rights/dk/atira/pure/core/openaccesspermission/open
dc.subjectBioengineering
dc.subjectMaterials Chemistry
dc.subjectPolymers and Plastics
dc.subjectBiomaterials
dc.titleCLSM method for the dynamic observation of pH change within polymer matrices for oral deliveryen
dc.contributor.institutionDepartment of Pharmacy
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Accepted Version
rioxxterms.versionAM
rioxxterms.versionofrecordhttps://doi.org/10.1021/bm301569r
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeopenAccess


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